基质金属蛋白酶-8对角膜基质胶原的作用研究

发布时间：2018-07-31 14:15

【摘要】：目的探讨基质金属蛋白酶-8(MMP-8)对角膜的作用。方法选取健康C57BL/6J小鼠15只,右眼角膜基质内注射10μL MMP-8作为实验组,左眼给予等量生理盐水作为对照组。于0、4、8h使用双光子显微镜二次谐波成像技术对活体小鼠角膜逐层扫描,Imaris软件对所得图像三维重建,计算图像信号强度。4、8h在裂隙灯下评价角膜混浊度。8h角膜取材,测定各角膜羟脯氨酸水平。结果 0h实验组及对照组小鼠角膜基质纤维信号强度分别为89.7±11.2、85.3±7.0,4h分别为14.5±3.4、46.6±14.0,8h分别为11.0±4.6、34.6±12.5,4h及8h,实验组较对照组角膜基质信号强度明显降低(P0.05);4h及8h,实验组较对照组角膜明显混浊(P0.05);8h测得实验组与对照组角膜羟脯氨酸水平分别为(0.433±0.090)、(0.590±0.133)μg/mg,实验组明显低于对照组(F=7.193,P=0.014)。结论 MMP-8对小鼠角膜基质胶原有明显的降解破坏作用,导致角膜透明度下降。
[Abstract]:Objective to investigate the effect of matrix metalloproteinase-8 (MMP-8) on cornea. Methods Fifteen healthy C57BL/6J mice were injected with 10 渭 L MMP-8 into the corneal stroma of the right eye as the experimental group, and the left eye was treated with the same amount of normal saline as the control group. Using two-photon microscope second harmonic imaging technique, the images were reconstructed by the software Imaris in vivo for 8 h. The image signal intensity was calculated for 8 h. The corneal turbidity was evaluated under slit lamp for 8 h. The levels of hydroxyproline in the cornea were measured. Results the signal intensities of corneal stromal fibers in the experimental group and the control group were 89.7 卤11.2U 85.3 卤7.0h and 14.5 卤3.4g 46.6 卤14.0h, respectively, which were 11.0 卤4.6 卤12.5h and 8h, respectively. The signal intensity of corneal stroma in the experimental group was significantly lower than that in the control group (P0.05) for 4h and 8h. The corneal signal intensity in the experimental group was significantly lower than that in the control group (P0.05) and the corneal signal intensity in the experimental group was significantly higher than that in the control group. The corneal hydroxyproline levels in the experimental group and the control group were (0.433 卤0.090), (卤0.590 卤0.133) 渭 g / mg, respectively, which were significantly lower than those in the control group (P 0.05). Conclusion MMP-8 can degrade and destroy the collagen of corneal stroma and decrease the transparency of cornea.
【作者单位】：郑州大学人民医院眼科/河南省眼科研究所/河南省立眼科医院;锦州医科大学眼科学;新乡医学院;
【基金】：河南省自然科学基金资助项目(162300410163) 河南省创新人才计划项目(144100510015) 河南省国际合作项目(152102410085)
【分类号】：R772.21

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