大鼠外侧膝状体神经元时空感受野特性研究
发布时间：2018-08-04 18:28
【摘要】：目的 观察大鼠外侧膝状体(LGN)神经元感受野时空频率调谐特性的发育变化以及异常视觉经验(单眼形觉剥夺)对LGN神经元时空频率调谐及局部震荡特性的影响。 方法 1.睁眼后14-16d组、睁眼后20-22d组、睁眼后27-30d组、睁眼后60d组Wistar大鼠各5只,用在体细胞外记录技术,正弦光栅视觉刺激检测各组大鼠LGN神经元感受野时空调谐特性,观察大鼠LGN神经元时空频率调谐特性发育之动态变化。 2.单眼形觉剥夺Wistar大鼠10只,用在体细胞外记录技术,正弦光栅视觉刺激,观察LGN剥夺层与非剥夺层神经元感受野时空频率调谐特性及局部震荡特性的变化。 结果 1.睁眼后14-16d组、睁眼后20-22d组、睁眼后27-30d组、睁眼后60d组大鼠LGN神经元时间和空间频率调谐低通和带通间的分布差异均无统计学意义(χ2＝0.68,0.47, P0.05),4个组大鼠LGN神经元感受野最优时间频率调谐特性成年后达最高值,最优时间频率均值分别为(2.6±1.5)、(2.6±1.5)、(2.5±1.5)、(3.6±2.3) cycles/s,差异有统计学意义(F=3.4,P0.05),睁眼后60d组的最优时间频率明显高于睁眼后14-16d组、睁眼后20～22d组、睁眼后27-30d组,差异有统计学意义(q=4.43,4.10,4.03,P0.05),余3组间的相互比较差异均无统计学意义(P0.05)。四组瞬时带宽均值在1.7±1.9octaves,各年龄组之间比较,差异无统计学意义(F=0.22,P0.05)。4组大鼠LGN神经元的最优空间频率(中值)分别为0.03、0.03、0.035、0.04cycles/deg,空间分辨率为(0.26士0.15)、(0.26±0.15)、(0.28±0.13)、(0.29±0.14) cycles/deg,空间带宽分别(2.7士1.2)、(2.8±1.2)、(3.0±1.0)、(2.4±1.0)octaves,上述三项指标差异均无统计学意义(F=0.34、1.23、0.50,P0.05)。 方向敏感性细胞、方位敏感性细胞及无方向选择性细胞比例各年龄组分布大致相同：方向选择性细胞约占五分之一,方位选择性细胞约占三分之二,余为方向选择性不敏感细胞(x2=0.26,P0.05)。随着年龄的增长,LGN神经元对比度阈值逐渐降低,成年前三组分别为(31.5±17.8)%、(29.1±16.6)%、(28.2±18.4)%,到成年60d时达到最低(19.4±17.5)%,较成年前3组间比较差别具有统计学意义(F＝3.2,P0.05,q=11.98.8.30.7.90),成年前3组间差别无统计学意义(P0.05)。 2.LGN神经元时空间频率调制细胞数与非调制细胞数各组之间差别弱剥夺组与非剥夺组之间比较差异无统计学意义(χ2=0.00,P0.05)。剥夺层神经元感受野的最优时间频率为(2.5±1.4)cycles/s,非剥夺层支配LGN细胞最优时间频率为(2.5±1.3)cycles/s,剥夺组与非剥夺组差别无统计学意义(t=0.013,P0.05)；剥夺层局部场电位(LFP) γ-波段(25-90赫兹)功率谱为(11.65±4.72)V^2*e-009,非剥夺层γ-波段功率谱为(14.09±3.90) V^2*e-009,剥夺组明显低于非剥夺组,差别具有统计学意义((t=2.93,P0.05)。 剥夺层神经元感受野最优空间频率为0.03(中值)cycles/deg,空间分辨率为(0.26±0.14)cycles/deg,非剥夺层最优空间频率0.03(中值)cycles/deg,空间分辨率为(0.27±0.13)cycles/deg,最优空间频率、空间分辨率二者之间差别均无统计意义(χ2=0.34,t＝1.23,P0.05)；剥夺层LFP Y-波段功率谱为(16.73±7.34)V^2*e-009,非剥夺层γ-波段功率谱为(7.27±3.0)V^2*e-009,剥夺组明显低于非剥夺组,差别具有统计学意义(t=8.5,P0.05)。 结论 1.睁眼后14-14d、睁眼后20-22d、睁眼后27～30d、睁眼后60d大鼠LGN神经元感受野空间频率、方位和方向选择性调谐特性在睁眼后2天即已发育成熟。时间频率、对比度,至成年才成熟。时空频率调谐发育特性的不同可能与其在视觉通路中的功能有关。 2.单眼形觉剥夺不改变大鼠LGN神经元时空感受野特性,但局部神经元集群处理时空信息的活动明显减弱,单眼形觉剥夺影响大鼠LGN神经元集群活动,为研究弱视发生机制提供了一个新的线索。
[Abstract]:objective
The changes in the spatial and temporal tuning characteristics of the neurons of the lateral geniculate (LGN) neurons in the rat lateral geniculate body and the effects of abnormal visual experience (monocular form deprivation) on the temporal and spatial frequency tuning and local oscillation of LGN neurons were observed.
Method
1. after opening eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, 5 Wistar rats in group 60d after opening eyes, using the technique of extracellular recording, and sinusoidal grating visual stimulation to detect the temporal and spatial tuning characteristics of LGN neurons in the rats of each group, and observe the dynamic changes of the time and space frequency tuning characteristics of LGN neurons in the rat LGN.
2. the 10 Wistar rats with monocular form deprivation were used in the extracellular recording technique and sinusoidal grating visual stimulation to observe the frequency tuning and local oscillation characteristics of the LGN deprivation and non deprived neurons in the receptive field.
Result
1. after open eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, LGN neurons in group 60d after opening eyes, LGN neuron time and space frequency tuning low pass and the distribution difference between the band is not statistically significant (x 2 = 0.68,0.47, P0.05), the optimal time frequency tuning characteristic of the 4 groups of rat LGN neurons is the highest, the optimal time frequency is the highest, the optimal time frequency The average rate was (2.6 + 1.5), (2.6 + 1.5), (2.5 + 1.5), (3.6 + 2.3) cycles/s, and the difference was statistically significant (F=3.4, P0.05). The optimal time frequency of group 60d was significantly higher than that of group 14-16d after open eyes, and group 20 to 22d after opening eyes, and the difference was statistically significant (q=4.43,4.10,4.03, P0.05) after opening eyes (q=4.43,4.10,4.03, P0.05), and the differences of the remaining 3 groups were all different There was no statistical significance (P0.05). The mean instantaneous bandwidth in the four group was 1.7 + 1.9octaves, and the difference was not statistically significant (F=0.22, P0.05), the optimal spatial frequency (median) of LGN neurons in the.4 group was 0.03,0.03,0.035,0.04cycles/deg, the spatial differentiation rate was (0.26 0.15), (0.26 + 0.15), (0.28 + 0.13), (0.29 + 0.14) Cycl ES / deg, spatial bandwidth were (2.7s 1.2), (2.8 + 1.2), (3.0 + 1.0), (2.4 + 1.0) octaves, respectively. There was no significant difference among the three indexes (F = 0.34, 1.23, 0.50, P 0.05).
The proportion of direction sensitive cells, azimuth sensitive cells and non directional selective cells was approximately the same in all age groups: the direction selective cells accounted for about 1/5, azimuth selective cells accounted for about 2/3, and the other was directional selective insensitive cells (x2=0.26, P0.05). As the age increased, the contrast threshold of LGN neurons decreased gradually. The three groups in the first three groups were (31.5 + 17.8)%, (29.1 + 16.6)% and (28.2 + 18.4)%, reaching the lowest (19.4 + 17.5)% to adult 60d. The difference was statistically significant compared with the 3 groups (F = 3.2, P0.05, q=11.98.8.30.7.90), and there was no statistically significant difference between the three groups before adulthood (P0.05).
The difference between the number of spatial frequency modulation cells and the number of non modulation cells in 2.LGN neurons was not statistically significant (x 2=0.00, P0.05). The optimal time frequency of the deprivation neurons was (2.5 + 1.4) cycles/s, and the optimal time frequency of the non deprived LGN cells was (2.5 + 1.3) cycles/s. There was no significant difference between the deprivation group and the non deprived group (t=0.013, P0.05); the power spectrum of the partial field potential (LFP) gamma band (25-90 Hz) of the deprivation layer was (11.65 + 4.72) V^2*e-009, the non deprivation layer gamma band power spectrum was (14.09 + 3.90) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group (t=2.93, P0.05).
The optimal spatial frequency of the deprivation neurons was 0.03 (median) cycles/deg, the spatial resolution was (0.26 + 0.14) cycles/deg, the optimal spatial frequency of the non deprivation layer was 0.03 (median) cycles/deg, the spatial resolution was (0.27 + 0.13) cycles/deg, the optimal spatial frequency and the space resolution two had no statistical significance (x 2=0.34, t = 1.23, P0.0). 5) the power spectrum of the LFP Y- band of the deprivation layer was (16.73 + 7.34) V^2*e-009, the non deprivation layer gamma band power spectrum was (7.27 + 3) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group. The difference was statistically significant (t=8.5, P0.05).
conclusion
1. after opening eyes, 14-14d, 20-22d after opening eyes, 27 to 30d after opening eyes, and after opening eyes, LGN neurons of 60d rats feel the spatial frequency of field. The selective tuning characteristics of azimuth and direction are mature at 2 days after opening eyes. Time frequency, contrast, mature. The difference of temporal and spatial frequency tuning developmental characteristics may be related to its function in the visual pathway Close.
2. monocular form deprivation does not change the space-time receptive field characteristics of LGN neurons in rats, but the activity of the local neurons in the processing of spatio-temporal information is obviously weakened. Monocular form deprivation affects the activity of LGN neurons in rats, and provides a new clue for the study of the mechanism of amblyopia.
【学位授予单位】：天津医科大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R777.44
本文编号：2164732
[Abstract]:objective
The changes in the spatial and temporal tuning characteristics of the neurons of the lateral geniculate (LGN) neurons in the rat lateral geniculate body and the effects of abnormal visual experience (monocular form deprivation) on the temporal and spatial frequency tuning and local oscillation of LGN neurons were observed.
Method
1. after opening eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, 5 Wistar rats in group 60d after opening eyes, using the technique of extracellular recording, and sinusoidal grating visual stimulation to detect the temporal and spatial tuning characteristics of LGN neurons in the rats of each group, and observe the dynamic changes of the time and space frequency tuning characteristics of LGN neurons in the rat LGN.
2. the 10 Wistar rats with monocular form deprivation were used in the extracellular recording technique and sinusoidal grating visual stimulation to observe the frequency tuning and local oscillation characteristics of the LGN deprivation and non deprived neurons in the receptive field.
Result
1. after open eyes, group 14-16d, group 20-22d after opening eyes, group 27-30d after opening eyes, LGN neurons in group 60d after opening eyes, LGN neuron time and space frequency tuning low pass and the distribution difference between the band is not statistically significant (x 2 = 0.68,0.47, P0.05), the optimal time frequency tuning characteristic of the 4 groups of rat LGN neurons is the highest, the optimal time frequency is the highest, the optimal time frequency The average rate was (2.6 + 1.5), (2.6 + 1.5), (2.5 + 1.5), (3.6 + 2.3) cycles/s, and the difference was statistically significant (F=3.4, P0.05). The optimal time frequency of group 60d was significantly higher than that of group 14-16d after open eyes, and group 20 to 22d after opening eyes, and the difference was statistically significant (q=4.43,4.10,4.03, P0.05) after opening eyes (q=4.43,4.10,4.03, P0.05), and the differences of the remaining 3 groups were all different There was no statistical significance (P0.05). The mean instantaneous bandwidth in the four group was 1.7 + 1.9octaves, and the difference was not statistically significant (F=0.22, P0.05), the optimal spatial frequency (median) of LGN neurons in the.4 group was 0.03,0.03,0.035,0.04cycles/deg, the spatial differentiation rate was (0.26 0.15), (0.26 + 0.15), (0.28 + 0.13), (0.29 + 0.14) Cycl ES / deg, spatial bandwidth were (2.7s 1.2), (2.8 + 1.2), (3.0 + 1.0), (2.4 + 1.0) octaves, respectively. There was no significant difference among the three indexes (F = 0.34, 1.23, 0.50, P 0.05).
The proportion of direction sensitive cells, azimuth sensitive cells and non directional selective cells was approximately the same in all age groups: the direction selective cells accounted for about 1/5, azimuth selective cells accounted for about 2/3, and the other was directional selective insensitive cells (x2=0.26, P0.05). As the age increased, the contrast threshold of LGN neurons decreased gradually. The three groups in the first three groups were (31.5 + 17.8)%, (29.1 + 16.6)% and (28.2 + 18.4)%, reaching the lowest (19.4 + 17.5)% to adult 60d. The difference was statistically significant compared with the 3 groups (F = 3.2, P0.05, q=11.98.8.30.7.90), and there was no statistically significant difference between the three groups before adulthood (P0.05).
The difference between the number of spatial frequency modulation cells and the number of non modulation cells in 2.LGN neurons was not statistically significant (x 2=0.00, P0.05). The optimal time frequency of the deprivation neurons was (2.5 + 1.4) cycles/s, and the optimal time frequency of the non deprived LGN cells was (2.5 + 1.3) cycles/s. There was no significant difference between the deprivation group and the non deprived group (t=0.013, P0.05); the power spectrum of the partial field potential (LFP) gamma band (25-90 Hz) of the deprivation layer was (11.65 + 4.72) V^2*e-009, the non deprivation layer gamma band power spectrum was (14.09 + 3.90) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group (t=2.93, P0.05).
The optimal spatial frequency of the deprivation neurons was 0.03 (median) cycles/deg, the spatial resolution was (0.26 + 0.14) cycles/deg, the optimal spatial frequency of the non deprivation layer was 0.03 (median) cycles/deg, the spatial resolution was (0.27 + 0.13) cycles/deg, the optimal spatial frequency and the space resolution two had no statistical significance (x 2=0.34, t = 1.23, P0.0). 5) the power spectrum of the LFP Y- band of the deprivation layer was (16.73 + 7.34) V^2*e-009, the non deprivation layer gamma band power spectrum was (7.27 + 3) V^2*e-009, and the deprivation group was significantly lower than that of the non deprived group. The difference was statistically significant (t=8.5, P0.05).
conclusion
1. after opening eyes, 14-14d, 20-22d after opening eyes, 27 to 30d after opening eyes, and after opening eyes, LGN neurons of 60d rats feel the spatial frequency of field. The selective tuning characteristics of azimuth and direction are mature at 2 days after opening eyes. Time frequency, contrast, mature. The difference of temporal and spatial frequency tuning developmental characteristics may be related to its function in the visual pathway Close.
2. monocular form deprivation does not change the space-time receptive field characteristics of LGN neurons in rats, but the activity of the local neurons in the processing of spatio-temporal information is obviously weakened. Monocular form deprivation affects the activity of LGN neurons in rats, and provides a new clue for the study of the mechanism of amblyopia.
【学位授予单位】：天津医科大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R777.44
【参考文献】
相关期刊论文 前1条
1 ;Effects of age on latency and variability of visual response in monkeys[J];Chinese Science Bulletin;2005年11期
，本文编号：2164732
本文链接：https://www.wllwen.com/yixuelunwen/wuguanyixuelunwen/2164732.html
最近更新
教材专著
