七氟醚预处理对梗阻性黄疸肝缺血再灌注大鼠肾损伤的影响及机制

发布时间：2018-01-08 03:29

本文关键词：七氟醚预处理对梗阻性黄疸肝缺血再灌注大鼠肾损伤的影响及机制　出处：《山东医药》2016年12期 　论文类型：期刊论文

【摘要】：目的观察七氟醚对梗阻性黄疸肝缺血再灌注大鼠肾损伤的影响,探讨其作用机制。方法将60只SD大鼠随机分成三组,每组20只。模型组制备梗阻性黄疸肝缺血再灌注模型;七氟醚预处理组吸入七氟醚30 min、吸入空气30 min,制备梗阻性黄疸肝缺血再灌注模型;正常对照组仅手术游离肝门至胆总管。分别于处理1、3、6、12、24 h每组随机选取4只大鼠行下腔静脉采血,检测血清肌酐(Cr)、尿素氮(BUN)。处死大鼠,取左肾组织,光镜下观察肾组织病理学变化;制备肾组织匀浆,检测肾组织丙二醛(MDA)、超氧化物歧化酶(SOD)、三磷酸腺苷(ATP)。结果对照组不同时间点肾小管及肾间质结构正常;模型组肾脏近曲小管上皮细胞浊肿,并出现细胞崩析、广泛细胞管形成;七氟醚预处理组肾组织改变同模型组,但程度均减轻。与对照组比较,模型组及七氟醚预处理组各时间点血清Cr、BUN水平均升高(P均0.01);七氟醚预处理组各时间点血清Cr和BUN水平均低于模型组(P0.05或0.01)。与对照组比较,模型组及七氟醚预处理组各时间点肾组织MDA升高,SOD、ATP降低,组间比较P均0.01;七氟醚预处理组各时间点肾组织MDA低于模型组,SOD、ATP高于模型组,组间比较P0.05或0.01。结论七氟醚预处理可减轻梗阻性黄疸肝缺血再灌注大鼠肾损伤,其机制可能与减少氧自由基产生有关。
[Abstract]:Objective to observe the effect of sevoflurane on renal injury in rats with obstructive jaundice liver ischemia reperfusion. Methods 60 SD rats were randomly divided into three groups. 20 rats in each group. The model group was used to establish hepatic ischemia-reperfusion model of obstructive jaundice. Sevoflurane preconditioning group inhaled sevoflurane for 30 min and inhaled air for 30 min to establish hepatic ischemia-reperfusion model of obstructive jaundice. The normal control group only operated on free hepatic hilum to common bile duct. 4 rats in each group were randomly selected to collect blood from the inferior vena cava for 24 h after treatment of 1: 3, 6, 12 and 24 h, and the serum creatinine (Cr) was detected. The rats were killed and left renal tissue was taken. The pathological changes of renal tissue were observed under light microscope. The renal homogenate was prepared, and the renal tissue malondialdehyde (MDA), superoxide dismutase (SOD) and adenosine triphosphate (ATP) were detected. Results the renal tubule and interstitial structure of the control group were normal at different time points. In the model group, the epithelial cells of proximal convoluted tubules in the kidney were turbid, the cells collapsed and a wide range of cell tubes were formed. The changes of renal tissue in sevoflurane preconditioning group were similar to those in model group, but the degree was reduced. Compared with control group, the serum Cr bun levels in model group and sevoflurane pretreatment group were increased at each time point (P < 0.01). The serum Cr and BUN levels in sevoflurane pretreatment group were lower than those in model group (P0.05 or 0.01). In model group and sevoflurane preconditioning group, MDA in renal tissue increased and decreased at each time point, compared with each other (P 0.01). The MDA of renal tissue in sevoflurane pretreatment group was lower than that in model group at each time point. Conclusion Preconditioning with sevoflurane can reduce renal injury in rats with obstructive jaundice after hepatic ischemia reperfusion, and its mechanism may be related to reducing the production of oxygen free radicals.
【作者单位】：中山大学附属第五医院;
【基金】：珠海市科技计划项目(2014D0401990017)
【分类号】：R575
【正文快照】： 肝脏缺血再灌注损伤是梗阻性黄疸手术中常见的病理生理过程,其产生原因与原发疾病本身及手术方式等有关,可导致肝脏本身及远隔脏器(如肾脏)的损伤[1~3]。氧化应激和炎症反应是缺血再灌注损伤的主要发病机制。麻醉剂七氟醚具有抗炎特性,但其对缺血再灌注损伤的影响尚不清楚。20

【相似文献】