白介素-7对CCL4诱导的小鼠肝纤维化作用及影响因素

发布时间：2018-01-08 18:00

本文关键词：白介素-7对CCL4诱导的小鼠肝纤维化作用及影响因素　出处：《南昌大学》2016年硕士论文　论文类型：学位论文

更多相关文章： 白介素-7 CCL4 肝纤维化 影响因素

【摘要】：目的探讨白介素-7对CCL4诱导的小鼠肝纤维化作用及影响因素。方法用四氯化碳(CCl4)诱导形成小鼠肝纤维化模型。将雌性balb/c小鼠36只随机分成3组:A组(生理盐水对照组),B组(纤维化模型组),C组(IL-7干预组),每组12只。每个组随机再分为两个亚组,分别记为A1、A2,B1、B2及C1、C2(1表示2周,2表示4周),每亚组6只。A1、B1、C1组及A2、B2、C2组分别于实验2周和4周后取血并处死取肝脏,用ELASA法检测各组小鼠血清中ALT、AST浓度,肉眼及组织病理形态学法(常规HE染色、masson染色及网状纤维染色)观察肝组织标本,并采用Ishak半定量评分系统对小鼠肝组织炎症活动度及纤维化程度进行评分。用SPSS18.0统计软件对实验数据进行分析处理。结果成功建立小鼠肝纤维化模型,表现为汇管区及周围纤维扩大,有芒状纤维和小叶纤维间隔形成。血生化检测:同时期相比,B、C组ALT及AST均显著高于A组(P0.05),C组ALT及AST均显著低于B组(P0.05);同组内不同时间亚组比较:B2组AST显著高于B1组(P0.05),C2组ALT及AST均显著低于C1组(P0.05)。HE染色、Masson染色和网状纤维染色均显示:同时期相比,B组小鼠炎症及纤维化程度均最严重,C组较B组轻;亚组间比较:B2组炎症及纤维化程度均较B1组重,而C2组较C1组轻;Ishak炎症活动度及肝纤维化程度评分及统计学分析示:同时期三组相比,B组炎症及纤维化评分均最高,三组之间差异显著(P0.05),同组内亚组比较:B2炎症及纤维化评分均显著高于B1组(P0.05);C2组炎症评分及纤维化评分均低于C1组,炎症评分差异显著(P0.05),纤维化评分差异不明显(P0.05)。结论白介素-7能抑制CCl4诱导的小鼠肝纤维化,减轻肝脏炎症,作用效果与干预时间相关。
[Abstract]:Objective to investigate the effect of interleukin-7 (IL-7) on CCL4 induced liver fibrosis in mice and its influencing factors. 36 female balb/c mice were randomly divided into 3 groups: group A (normal saline control group). Group B (Fibrosis model group) treated with IL-7 group (12 rats in each group). Each group was randomly divided into two subgroups, which were marked as A1A2OB1B1B2 and C1C2O1 respectively for 2 weeks. (2) for 4 weeks, 6 rats in each subgroup. The blood was taken from the blood and the liver was killed in the group A _ 2 B _ 2C _ 2 and A _ (1) B _ (1) C _ (1) and A _ (2) B _ (2) C _ 2 after 2 and 4 weeks, respectively. The concentration of alt AST in serum, histopathology (routine HE staining, mass on staining and reticular fiber staining) were detected by ELASA method. Ishak semi-quantitative scoring system was used to evaluate the degree of inflammatory activity and fibrosis in liver tissue of mice. The experimental data were analyzed and processed by SPSS18.0 software. Results the mice were successfully established. Liver fibrosis model. The blood biochemical test showed that the ALT and AST of group C were significantly higher than that of group A (P 0.05). ALT and AST in group C were significantly lower than those in group B (P 0.05). The AST of group B 2 was significantly higher than that of group B1 (P 0.05) and AST of group C 2 was significantly lower than that of group C 1 (P 0.05). Masson staining and reticular fiber staining showed that the severity of inflammation and fibrosis in group B was lighter than that in group B at the same time. The degree of inflammation and fibrosis in group B _ 2 was heavier than that in group B1, while that in group C _ 2 was lighter than that in group C _ 1. Ishak inflammatory activity and liver fibrosis degree score and statistical analysis showed that: the same period of the three groups compared with the B group inflammation and fibrosis score were the highest, the difference between the three groups was significant (P 0.05). The score of inflammation and fibrosis in group B 2 was significantly higher than that in group B 1 (P 0.05). The inflammation score and fibrosis score of C2 group were lower than that of C1 group, and the difference of inflammation score was significant (P0.05). Conclusion Interleukin-7 can inhibit CCl4 induced liver fibrosis and alleviate liver inflammation in mice. The effect is related to the time of intervention.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R575.2

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