Helicobacter suis感染小鼠模型的建立及其意义

发布时间：2018-01-11 19:16

本文关键词：Helicobacter suis感染小鼠模型的建立及其意义　出处：《中国人兽共患病学报》2016年07期 　论文类型：期刊论文

【摘要】：目的建立Helicobacter suis(H.suis)感染小鼠模型并探究其意义。方法将40只雌性C57BL/6小鼠随机分为模型组和对照组,模型组给予0.5mL猪胃黏膜匀浆液(经PCR检测确定存在H.suis)灌胃,对照组给予0.5mL PBS溶液灌胃。灌胃1个月及3个月后,各处死两组中半数小鼠,取其胃黏膜组织,分别行PCR、HE染色检测H.suis在小鼠胃内的定植与胃黏膜淋巴滤泡形成情况。结果灌胃所用猪胃黏膜匀浆液中存在H.suis;H.suis可以在小鼠胃内定植,并且3个月后仍存在;感染H.suis 1个月及3个月后的小鼠胃黏膜均有淋巴滤泡的形成,并且后者的淋巴滤泡较前者明显增大。结论成功建立了H.suis感染C57BL/6小鼠模型,H.suis可以诱导胃黏膜淋巴滤泡的形成,可能在胃黏膜相关淋巴组织(MALT)淋巴瘤的发生过程中发挥重要作用。
[Abstract]:Objective to establish Helicobacter suis H. suis. Methods 40 female C57BL / 6 mice were randomly divided into model group and control group. The model group was given 0.5 mL gastric mucosal homogenate (determined by PCR), while the control group was given 0.5 mL PBS solution for 1 month and 3 months later. Half of the mice died in each group, their gastric mucosal tissues were taken and PCR was performed respectively. He staining was used to detect H. suis colonization and gastric mucosal lymphatic follicle formation in mice. Results H. suis was found in pig gastric mucosa homogenate. H. suis could be colonized in the stomach of mice and still existed after 3 months. Lymphatic follicles were found in gastric mucosa of mice infected with H. suis for 1 and 3 months. Conclusion H.suis infected C57BL / 6 mouse model can induce gastric mucosal lymphoid follicle formation. It may play an important role in the development of gastric mucosal associated lymphoid tissue malt lymphoma.
【作者单位】：青岛大学附属医院消化内科;
【基金】：国家自然科学基金面上项目(No.81572320)资助~~
【分类号】：R-332;R573
【正文快照】： (Department of Gastroenterology,the Affiliated Hospital of Qingdao University,Qingdao 266003,China)Supported by the National Natural Science Foundation of China(No.81572320)Corresponding author:Yang Lin,Email:bobotony@126.com海尔曼螺杆菌(Helicobacter hei

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