HBV慢性感染者不同阶段的特异性CTLs水平分析

发布时间：2018-02-04 02:15

本文关键词： 乙肝病毒慢性感染细胞毒性T淋巴细胞酶联免疫斑点试验　出处：《免疫学杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的探讨乙肝病毒(HBV)慢性感染者疾病不同阶段的特异性细胞毒性T淋巴细胞(CTLs)水平及其临床意义。方法本研究共收集乙肝病毒慢性感染患者81例,表面抗体和核心抗体阳性的正常对照20例,依据HBV感染后的自然史将慢性HBV感染者分为免疫耐受期患者(耐受组)、免疫清除期患者(活动组)和非活动期患者(非活动组),采用酶联免疫斑点试验(ELISPOT)检测患者外周血中分泌IFN-γ的特异性CTLs数量,并分析其与临床指标的相关性。结果慢性HBV感染者的特异性CTLs反应较弱,其反应强度和阳性率均明显低于正常对照组(P0.05)。对于不同疾病分期的慢性HBV感染者,CTLs数量在免疫耐受组非活动组活动组,差异有统计学意义(P0.05)。相关性分析显示,免疫清除期患者的表面抗原和核心抗原特异性CTLs数量与血清ALT之间呈显著正相关(P0.05)。结论慢性HBV感染者不同阶段的特异性免疫反应存在明显差异,对于免疫清除期患者,血清ALT在一定程度上反应了HBV特异性CTLs反应的强度。
[Abstract]:Objective to investigate the specific cytotoxic T lymphocyte CTLs in chronic hepatitis B virus (HBV) infected patients at different stages. Methods 81 patients with chronic hepatitis B virus infection were collected. According to the natural history of HBV infection, chronic HBV infected patients were divided into immune tolerance group (tolerance group). Immune clearance patients (active group) and inactive patients (inactive group). The specific CTLs levels of IFN- 纬 secreted in peripheral blood of patients were detected by Elisa dot test (Elisa). Results the specific CTLs response of chronic HBV infected patients was weak. The response intensity and positive rate were significantly lower than those in the normal control group (P 0.05). The number of CTLs in chronic HBV infected patients with different disease stages was significantly lower than that in the inactive group of immune tolerance group. The difference was statistically significant (P 0.05). Correlation analysis showed. There was a significant positive correlation between the number of surface antigen and core antigen specific CTLs and serum ALT in patients with immune clearance (P 0.05). Conclusion there are significant differences in specific immune responses in different stages of chronic HBV infection. In patients with immune clearance, serum ALT reflects the intensity of HBV specific CTLs reaction to some extent.
【作者单位】：川北医学院附属医院检验科;北京首都医科大学附属北京佑安医院感染与免疫研究中心;
【基金】：北京市自然科学基金(7111004)
【分类号】：R512.62
【正文快照】： 我国乙肝病毒(hepatitis B virus,HBV)感染者约9 300万,慢性乙型肝炎患者约2 000万例[1]。HBV慢性感染的自然史一般可人为地划分为4个期[1-3],即免疫耐受期、免疫清除期、非活动或低(非)复制期和再活动期,各期患者的机体特异性免疫特征、病毒复制水平和临床表现等均明显不同。

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