ELISA法检测抗-HCV-IgG抗体以及FQ-PCR检测血清HCV-RNA在丙型肝炎诊断中的应用价值

发布时间：2018-02-04 13:58

  本文关键词： 丙型肝炎病毒 ELISA FQ-PCR ALT AST　出处：《广东医学》2015年24期 　论文类型：期刊论文

【摘要】：目的探讨酶联免疫吸附试验(ELISA)法检测抗-HCV-IgG抗体以及实时荧光定量聚合酶链反应(FQ-PCR)检测血清HCV-RNA在丙型肝炎诊断中的应用价值。方法对70例肝病科及肾病科丙型肝炎病毒(HCV)待查者血清标本,同时采用ELISA法检测抗-HCV-IgG抗体滴度,FQ-PCR法检测HCV-RNA载量,全自动生化分析仪检测ALT、AST,分析不同检测结果组合类型的HCV-RNA载量或抗-HCV-IgG抗体滴度与ALT、AST、AST/ALT值的关系。结果在70例血清标本中,用ELISA法检测到抗-HCV-IgG(+)(S/C.O.值≥1)有49例,使用FQ-PCR法检测到HCV-RNA(+)(载量≥5.0×102IU/m L)有51例。男女之间感染率差异无统计学意义(P0.05),但不同年龄组感染率差异有统计学意义(P0.05)。单阳性型患者与双阳性型患者的血清ALT异常率、AST异常率或AST/ALT倒置率(AST/ALT1)差异均无统计学意义(均P0.05)。HCV-RNA(+)且抗-HCV-IgG(+)患者的lgHCV-RNA载量与抗-HCV-IgG抗体S/C.O.值、ALT水平均呈线性正相关(r值分别为0.586、0.521,均P0.05),但是与AST/ALT值不呈线性相关(r=-0.076,P0.05)。HCV-RNA(+)且抗-HCV-IgG(-)患者的lg HCV-RNA载量与ALT水平、AST/ALT值均无直线相关关系存在(r值分别为-0.333、0.611,均P0.05)。HCV-RNA(-)且抗-HCV-Ig G(+)患者的抗-HCV-IgG抗体S/C.O.值与ALT水平、AST/ALT值也无直线相关关系存在(r值分别为0.485、0.563,均P0.05)。结论单一使用ELISA法检测抗-HCV-IgG抗体或FQ-PCR法检测HCV-RNA,对于丙型肝炎的诊断都有一定局限性。同时应用ELISA法和FQ-PCR法进行检测时,不同结果组合类型的患者,其HCV-RNA载量或抗-HCV-IgG抗体S/C.O.值与ALT水平、AST/ALT值的相关性并不一致。
[Abstract]:Objective to study the detection of anti-HCV-IgG antibody and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) by Elisa. Methods the serum samples of 70 patients with hepatitis C virus in hepatology and nephrology were studied. At the same time, ELISA method was used to detect the titer of anti-HCV-IgG antibody and FQ-PCR method was used to detect HCV-RNA load, and automatic biochemical analyzer was used to detect alt. The relationship between the HCV-RNA load or anti-HCV-IgG antibody titer and the alt value of alt was analyzed. The results were obtained in 70 serum samples. There were 49 cases of anti-HCV-IgG (S / C. O. Value 鈮，

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