CXCL8及其受体CXCR1、CXCR2在慢性乙肝患者外周血PMNs中的表达

发布时间：2018-02-05 17:24

本文关键词： 慢性乙肝中性粒细胞(PMNs) CXCL CXCR CXCR 免疫细胞化学染色卵白素-生物素复合物法　出处：《中国免疫学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的:探讨慢性乙肝患者外周血中性粒细胞(PMNs)上CXCL8及其受体CXCR1、CXCR2的表达。方法:以中性粒细胞分离液分离、纯化PMNs,检测患者血清HBe Ag及PMNs内HBV DNA,入选患者依据检测结果进行分组,SABC免疫细胞化学染色法检测各组患者PMNs内CXCL8及其受体CXCR1、CXCR2的表达。结果:SABC免疫细胞化学染色结果显示,CXCL8主要位于PMNs胞浆中,CXCR1、CXCR2多见于胞浆和胞膜上。其中HBe Ag(+)者CXCL8、CXCR1免疫着色较深,而CXCR2免疫着色较浅;PMNs内HBV DNA(+)者CXCL8、CXCR1免疫着色亦较深,而CXCR2免疫着色亦较浅。患者CXCL8和CXCR1的水平均显著升高,与正常对照相比,差异均有显著性(P0.05),而CXCR2的表达无统计学意义(P0.05)。结论:HBV侵染中性粒细胞后可促进CXCL8分泌,使胞膜CXCR1表达进一步增强。CXCL8、CXCR1、CXCR2免疫组化染色程度与患者HBe Ag表达、HBV DNA载量密切相关。高表达CXCR1的中性粒细胞与CXCL8相互作用,趋化吸引更多PMNs至病灶,参与局部炎性损伤和组织修复。
[Abstract]:Objective: to investigate the expression of CXCL8 and its receptor CXCR1 / CXCR2 on peripheral blood neutrophils (PMNs) of patients with chronic hepatitis B. methods: neutrophils were isolated from PMNs. The serum HBe Ag and HBV DNA in PMNs were detected by purified PMNs. The patients were divided into groups according to the test results. SABC immunocytochemical staining method was used to detect the expression of CXCL8 and its receptor CXCR1 CXCR2 in PMNs of patients in each group. CXCL8 was mainly located in the cytoplasm of PMNs, CXCR1 and CXCR2 were found on the cytoplasm and membrane, and the CXCL8CXCR1 immunoreactive cells with HBe Ag1 () were more heavily stained. However, CXCR2 immunostaining was light. The immunostaining of CXCL8 and CXCR1 in HBV DNA () in PMNs was also deep. The levels of CXCL8 and CXCR1 in the patients were significantly higher than those in the normal controls (P 0.05). However, there was no significant difference in the expression of CXCR2 (P 0.05). Conclusion the expression of CXCR1 in the cell membrane can be further enhanced by CXCR2 infection with neutrophils, which can promote the secretion of CXCL8 and enhance the expression of CXCR1 in the cell membrane of the neutrophilic granulocyte. The degree of immunohistochemical staining of CXCR1 and CXCR2 was closely related to the expression of HBe Ag and the amount of HBV DNA. The high expression of CXCR1 in neutrophils interacted with CXCL8. Chemotaxis attracts more PMNs to focus and participates in local inflammatory injury and tissue repair.
【作者单位】：安徽理工大学医学院病原学与免疫学教研室;
【基金】：安徽省自然科学基金(No.1308085MH148;090413138) 安徽省教育厅自然科学基金重点项目(No.KJ2010A086)
【分类号】：R512.62

【相似文献】