乙醇对大鼠枯否细胞吞噬功能影响及机制

发布时间：2018-02-09 21:07

  本文关键词： 乙醇 枯否细胞(KCs) 吞噬功能 微丝 膜流动性　出处：《中国公共卫生》2017年12期 　论文类型：期刊论文

【摘要】：目的观察乙醇对体外培养枯否细胞(KCs)吞噬功能、微丝表达和膜流动性影响。方法经门静脉插管胶原酶灌流消化肝脏,密度梯度离心分离KCs;体外培养KCs中加入不同剂量乙醇(1、2、4 g/L)作用3、24 h,采用聚苯乙烯乳珠吞噬实验、荧光染色法和荧光光度法检测KCs的吞噬功能、微丝表达、肌动蛋白含量及膜流动性。结果 1、2、4 g/L乙醇组作用3、24 h KCs吞噬聚苯乙烯乳珠数量分别为(16.05±3.69)、(15.86±4.50)、(13.86±4.69)和(13.95±4.51)、(14.35±6.52)、(9.71±3.34)个,明显低于对照组[分别为(20.13±5.26)、(20.30±8.57)个](P0.05);4 g/L乙醇组(作用24 h)KCs吞噬功能明显低于1、2 g/L乙醇组(P0.05);各剂量乙醇组(作用3 h)KCs微丝排列紊乱,但肌动蛋白含量无明显变化;与对照组(5.85±1.13)比较,2、4 g/L乙醇组KCs膜流动性[(2.30±1.08)、(1.62±0.30)]明显降低(P0.05),呈剂量依赖性。结论乙醇可降低体外KCs的吞噬功能,其机制可能与乙醇致KCs膜流动性下降和微丝排列紊乱有关。
[Abstract]:Objective to observe the effects of ethanol on the phagocytosis, microfilament expression and membrane fluidity of Kupffer cells in vitro. KCswere isolated by density gradient centrifugation and treated with different dosages of ethanolol (1g / L, 2g / L) for 3h for 24 h. The phagocytosis of KCs was detected by fluorescence staining and fluorescence spectrophotometry, and the expression of microfilaments was detected by fluorescence staining and fluorescence spectrophotometry. Results the content of actin and membrane fluidity in the ethanol group were 16.05 卤3.69 KCs and 13.86 卤4.69, respectively, and 14.35 卤6.529.35 卤6.529.71 卤3.34 in the ethanol group (n = 1, n = 2, n = 2, n = 2, n = 4) and in the ethanol group (n = 3, 24 h), respectively, and the results showed that the content of actin and membrane fluidity were 15.86 卤4.50 and 14.35 卤6.529.71 卤3.34, respectively. The phagocytosis of KCs was significantly lower than that of the control group (20.13 卤5.26) (20.30 卤8.57), the phagocytosis of KCs was significantly lower in the ethanol group (24 h) than that in the control group (20. 13 卤5. 26 卤8. 57), but the content of actin was not significantly changed in each dose of ethanol group (3 h after exposure), and the phagocytosis of KCs was significantly lower than that in the 1 g / L ethanol group (P 0. 05). Compared with the control group (5.85 卤1.13), the fluidity of KCs membrane in 2g / L ethanol group [2.30 卤1.08 卤0.30] significantly decreased in a dose-dependent manner. Conclusion ethanol can reduce the phagocytic function of KCs in vitro, and the mechanism may be related to the decrease of KCs membrane fluidity and the disorder of microfilament arrangement induced by ethanol.
【作者单位】： 山西医科大学汾阳学院科技中心;山西医科大学肝病研究所;
【基金】：山西医科大学汾阳学院人才启动基金(1302)
【分类号】：R575
，

本文编号：1498863