HCVF蛋白和APC基因甲基化与慢性HCV感染的相关研究

发布时间：2018-02-09 22:22

本文关键词： 基因 APC 甲基化肝炎病毒属　出处：《中华疾病控制杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨丙型肝炎病毒(hepatitis C virus,HCV)F蛋白与多发性腺瘤样息肉病(adenomatous polyposis coli,APC)基因甲基化在慢性HCV感染中的关系。方法收集慢性HCV患者(chronic hepatitis patient,CHP)的血样,检测F抗体(F antibody,F-Ab)的阳性率并且分成两组(F-Ab(+)CHP组、F-Ab(-)CHP组),收集健康者的血样作为对照组;分离外周血单核细胞(peripheral blood mononuclear cells,PBMCs),分别用HCV Core/F蛋白刺激,72 h后,分别提取DNA,用二代测序检测APC基因的甲基化水平,分析各组APC基因甲基化程度的差异。结果APC基因甲基化程度在F-Ab(+)CHP组、F-Ab(-)CHP组及健康对照组三组间差异有统计学意义(F=185.185,P0.001),F-Ab(+)CHP组APC基因甲基化程度高于F-Ab(-)CHP组,健康对照组最低(均有P0.05);三组样本PBMC分别经Core/F蛋白体外刺激后,APC基因甲基化程度各组总体比较差异均有统计学意义(均有P0.05),Core蛋白与F蛋白共刺激组细胞APC基因甲基化程度高于F蛋白刺激组,Core蛋白刺激组为最低。结论在慢性HCV感染患者中,HCV F蛋白的产生能够影响APC基因甲基化程度。
[Abstract]:Objective to investigate the relationship between the methylation of adenomatous polyposis coliform polyposis (polyposis) gene methylation and chronic HCV infection in patients with hepatitis C. methods the blood samples of patients with chronic HCV were collected. The positive rate of F antibody F-Abb was detected and divided into two groups. The blood samples of healthy subjects were collected as control group, and peripheral blood monocytes were isolated and stimulated with HCV Core/F protein for 72 h. DNA was extracted and the methylation level of APC gene was detected by second generation sequencing. Results the methylation degree of APC gene was significantly higher in F-Ab185.185 APC gene methylation group than that in F-Ab-CHP group. The healthy control group was the lowest (P 0.05), and the three groups had significant difference in the methylation degree of Core/F gene after the stimulation of Core/F protein in vitro (P 0.05) and F protein co-stimulated APC gene A in the cells of the three groups (P 0.05 and F protein co-stimulation group respectively), the APC gene methylation degree of the three groups was significantly higher than that of the control group (P 0.05% and F protein respectively). Conclusion the production of HCV F protein can affect the methylation of APC gene in patients with chronic HCV infection.
【作者单位】：南京医科大学基础医学院生物化学与分子生物学系;南京军区疾病预防控制中心疾病预防控制所;南京医科大学第一附属医院感染病科;
【基金】：国家自然科学基金(81573213,81172724) 江苏省自然科学基金(BK20151089,BK20161059,BL2013021) 中国肝炎基金会天晴肝病研究基金(CFHPC20132071)
【分类号】：R512.63

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