乌司他丁对肠缺血再灌注损伤大鼠小肠细胞凋亡的影响

发布时间：2018-02-20 00:05

本文关键词： 缺血再灌注细胞凋亡小肠细胞乌司他丁大鼠　出处：《山东医药》2015年05期 　论文类型：期刊论文

【摘要】：目的探讨乌司他丁对肠缺血再灌注损伤大鼠的保护作用及其机制。方法将60只健康Wistar大鼠随机分为对照组(A组)、肠缺血再灌注后0 h组(B1组)、肠缺血再灌注后2 h组(B2组)、乌司他丁治疗肠缺血再灌注后0 h组(C1)、乌司他丁治疗肠缺血再灌注后2 h组(C2组)各12只。B1、B2、C1、C2组夹闭肠系膜上动脉(SMA)60 min制作肠缺血再灌注损伤模型,并于手术前经尾静脉B1、B2组各注入生理盐水2 m L,C1、C2组各注入乌司他丁5×104U/kg;A组仅分离SMA,不夹闭血管。取A、B1、B2、C1、C2各组大鼠的小肠组织,光镜下观察组织学改变,TUNEL法检测小肠细胞凋亡率。结果光镜观察发现,A组小肠黏膜基本正常;B1组小肠黏膜及绒毛排列轻度紊乱,无黏膜变性、坏死,出现上皮下间隙扩大,腺体轻度受损及毛细血管充血;B2组小肠绒毛水肿明显,黏膜及绒毛排列明显紊乱,黏膜上皮脱落呈现糜烂,腺体严重受损;以上变化在绒毛顶端尤为显著。C1、C2组小肠黏膜充血、水肿及上皮糜烂坏死的程度均较B1、B2组减轻。B1、B2组大鼠细胞凋亡率较A组升高(P均0.01)。C1组与B1组、C2组与B2组比较细胞凋亡率均降低(P均0.05)。结论乌司他丁可抑制小肠细胞凋亡,有助于减轻由于缺血再灌注引起的肠道损伤。
[Abstract]:Objective to investigate the protective effect of ulinastatin on intestinal ischemia-reperfusion injury in rats and its mechanism. Methods 60 healthy Wistar rats were randomly divided into control group (group A), intestinal ischemia reperfusion group (group B 1), intestinal ischemia reperfusion group (group B 1) and intestinal ischemia reperfusion group (group B 2). H group B 2 group, ulinastatin treatment group 0 h after intestinal ischemia reperfusion, and ulinastatin treatment group 2 h after intestinal ischemia reperfusion group (group C 2) 12 rats each group clamped superior mesenteric artery SMA-60 min to make intestinal ischemia-reperfusion injury model. Before operation, the small intestine tissues of rats in each group were injected with normal saline 2 mL / L C _ (1) C _ (2) and ulinastatin 5 脳 10 ~ (4) U / kg ~ (-1) 路kg ~ (-1) A, only SMAs were isolated and blood vessels were not clipped. The small intestine tissues of rats in each group were taken from AMB B _ (1) B _ (2) B _ (2) C _ (1) C _ (2) group. Results the intestinal mucosa in group A was basically normal and the villi in group B1 were slightly disarranged, no degeneration of mucosa, necrosis, and enlargement of epigastric space were observed by Tunel method under light microscope, and Tunel method was used to detect the apoptosis rate of small intestine in group B.Results in group A, the intestinal mucosa was basically normal, and the villi in group B1 were slightly disarranged. Small intestinal villi edema, mucosal and villus arrangement disorder, erosion of mucosal epithelium and serious damage of glands were observed in small intestine villi of group B2 with slight glandular damage and capillary hyperemia, and the above changes were especially significant at the top of villous villi. The degree of edema and epithelial erosion and necrosis were decreased compared with that of B1B _ 2 group. The apoptosis rate of B _ (1) B _ 2 group was higher than that of A group (P < 0.01). C _ 1 group and B _ 1 group C _ 2 group and B _ 2 group were lower than B _ 2 group. Conclusion Ulinastatin can inhibit small intestinal cell apoptosis. It helps to alleviate the intestinal injury caused by ischemia reperfusion.
【作者单位】：内蒙古医科大学第一附属医院;
【分类号】：R574

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