骨桥蛋白对大鼠小肠隐窝上皮细胞生物学性状的影响

发布时间：2018-02-21 03:55

本文关键词： 骨桥蛋白肠上皮细胞细胞增殖细胞迁移大鼠　出处：《复旦学报(医学版)》2016年05期 　论文类型：期刊论文

【摘要】：目的观察骨桥蛋白(osteopontin,OPN)对大鼠小肠隐窝上皮细胞(intestinal epithelial cells-6,IEC-6)分化、增殖、迁移等生物学性状的影响。方法分别采用相差显微镜、细胞贴壁率检测、噻唑蓝比色法、划痕实验、Western blot和ELISA法,观察不同浓度OPN对IEC-6细胞增殖、分化和迁移能力的影响,并从鸟氨酸脱羧酶(ornithine decarboxylase,ODC)表达变化和炎性反应角度,探讨其可能的作用机制。结果经不同浓度的OPN处理后,IEC-6细胞形态、贴壁率和增殖率均未见明显变化。OPN能促进IEC-6细胞的移行功能,且OPN浓度越高细胞迁移速度越快。与低浓度或高浓度OPN相比,适当浓度的OPN能促进IEC-6细胞内ODC1蛋白表达(0.1μg/mL和1.0μg/mL OPN)和细胞内炎性反应(0.05μg/mL OPN)。结论 OPN能促进IEC-6细胞的移行功能,从而促进损伤黏膜早期修复,其机制可能与特定浓度的OPN能促进细胞内ODC1蛋白的表达和损伤IEC-6细胞的炎性反应有关。
[Abstract]:Objective to observe the effects of osteopontinin (OPN) on the differentiation, proliferation and migration of intestinal epithelial cells-6 (IEC-6) in rat intestinal crypt epithelium. The effects of different concentrations of OPN on the proliferation, differentiation and migration of IEC-6 cells were observed by Western blot and ELISA methods. The changes of ornithine decarboxylase ODCs and the inflammatory response of IEC-6 cells were observed from the point of view of ornithine decarboxylase ODC. Results the morphology, adherent rate and proliferation rate of IEC-6 cells treated with different concentrations of OPN had no obvious change. OPN could promote the migration function of IEC-6 cells. The higher the concentration of OPN, the faster the cell migration. Compared with the low or high concentration of OPN, the appropriate concentration of OPN could promote the expression of ODC1 protein (0.1 渭 g / mL and 1.0 渭 g / mL) and the intracellular inflammatory response (0.05 渭 g / mL) in IEC-6 cells. Conclusion OPN can promote the migration function of IEC-6 cells. The mechanism may be related to the specific concentration of OPN can promote the expression of ODC1 protein and the inflammatory response of IEC-6 cells.
【作者单位】：复旦大学附属上海市第五人民医院普外科;复旦大学附属上海市第五人民医院消化内科;复旦大学附属上海市第五人民医院急诊科;
【基金】：上海市闵行区卫生局科研课题(2011MW01)~~
【分类号】：R574

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