孟鲁司特治疗小鼠嗜酸性粒细胞性胃肠炎疗效观察

发布时间：2018-02-21 11:55

本文关键词： 胃肠炎嗜酸性粒细胞性白三烯受体拮抗剂孟鲁司特嗜酸性粒细胞 IgE 主要碱性蛋白小鼠　出处：《山东医药》2017年01期 　论文类型：期刊论文

【摘要】：目的观察孟鲁司特治疗卵清蛋白(OVA)诱导的小鼠嗜酸性粒细胞性胃肠炎(EG)的疗效,并探讨其作用机制。方法 40只雌性Balb/c小鼠随机分为正常对照组8只和OVA处理组32只。OVA处理组小鼠腹腔注射OVA和Al(OH)3溶液行基础致敏及强化致敏,继之OVA灌胃激发胃肠道过敏。造模成功后,将OVA处理组再随机分为模型组、孟鲁司特组、强的松组、强的松+孟鲁司特组各8只。模型组及各药物治疗组继续给予OVA隔日灌胃激发胃肠反应,同时药物治疗组分别给予强的松、孟鲁司特及二者联合灌胃治疗。观察EG小鼠外周血嗜酸性粒细胞(EOS)计数变化,ELISA法检测小鼠血清IgE水平,HE染色观察小鼠胃肠黏膜组织病理学变化,免疫组化方法检测小鼠空肠组织主要碱性蛋白(MBP)表达。结果模型组小鼠外周血EOS计数、血清IgE水平较正常对照组明显升高(P均0.01);与模型组比较,各药物治疗组小鼠外周血EOS计数、血清IgE水平降低(P均0.05)。模型组小鼠空肠MBP表达较正常对照组明显增强,各给药组空肠MBP表达均较模型组减弱。模型组小鼠胃黏膜变薄、断裂、下层充血,黏膜上皮细胞近胃腔端腺上皮脱落,有炎症细胞浸润。肠组织上皮细胞水肿,并见局灶性坏死、脱落,有大量炎性细胞浸润。各给药组小鼠胃肠黏膜各层结构基本正常,仅见轻微充血及炎症细胞浸润,上皮细胞结构基本正常。结论孟鲁司特通过降低EG小鼠的外周血EOS计数、IgE水平及空肠组织MBP表达,从而实现对EG的治疗作用。
[Abstract]:Objective to observe the effect of montelukast on eosinophilic gastroenteritis (EGG) induced by ovalbumin (OVA) in mice. Methods Forty female Balb/c mice were randomly divided into normal control group (n = 8) and OVA treated group (n = 32) by intraperitoneal injection of OVA and Al(OH)3 solution. After the successful establishment of the model, the OVA treatment group was randomly divided into three groups: model group, montelukast group, prednisone group, and prednisone group. Prednisone Montelukast group (n = 8), model group (n = 8) and drug treatment group (n = 8) were given OVA every other day to induce gastrointestinal reaction, while the drug treatment group were given prednisone respectively. The changes of eosinophil count in peripheral blood of EG mice were observed. The level of serum IgE was detected by Elisa and HE staining was used to observe the histopathological changes of gastrointestinal mucosa in EG mice. Results the EOS count in peripheral blood and serum IgE level in the model group were significantly higher than those in the normal control group (P < 0.01), and compared with the model group, the expression of the main basic protein in jejunum of the model group was determined by immunohistochemical method. The expression of MBP in jejunum of model group was significantly higher than that of normal control group, and the expression of MBP in jejunum of model group was lower than that of model group. The gastric mucosa of model group became thinner and ruptured. Hyperemia in the lower layer, exfoliation of mucosal epithelial cells near the gastric lumen, infiltration of inflammatory cells, edema of intestinal epithelial cells, focal necrosis and exfoliation, There were a large number of inflammatory cell infiltration. The gastrointestinal mucosa layer structure of mice in each group was basically normal, only slight congestion and inflammatory cell infiltration were observed. Conclusion Montelukast has a therapeutic effect on EG by decreasing the level of EOS count in peripheral blood and the expression of MBP in jejunum of EG mice.
【作者单位】：烟台毓璜顶医院;
【基金】：烟台市科技计划项目(2014WS018)
【分类号】：R57

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