脾脏免疫细胞在胃溃疡期间的变化及作用

发布时间：2018-02-24 06:33

本文关键词： 胃溃疡树突细胞巨噬细胞 T细胞免疫组化　出处：《中国老年学杂志》2015年11期 　论文类型：期刊论文

【摘要】：目的探讨实验性胃溃疡大鼠脾脏树突细胞(DC)、巨噬细胞(Mφ)和T细胞的变化和作用。方法胃前壁胃黏膜下注射冰乙酸制备大鼠胃溃疡模型,免疫组织化学检测正常组(5只)、盐水组(28只)和溃疡组(35只)雄性大鼠脾脏DC、Mφ和T细胞的变化。结果溃疡组、盐水组和正常组相比,DC、Mφ和T细胞数量均增多。溃疡2~10 d组DC数密度值逐渐增多,14 d后其值略有降低,其中6 d其值与盐水组相比差异显著(P0.05),6~14 d各组其值与正常组相比差异显著(P0.05,P0.01);溃疡组2~4 d组Mφ数密度值逐渐增多,6~14 d其值逐渐降低,各组其值均高于盐水组和正常组(P0.05,P0.01);溃疡组2~6 d T细胞数密度值持续增多,10 d后其值逐渐降低,其中4~10 d组其值与正常组相比差异显著(P0.01)。结论 DC和T细胞在溃疡期间脾脏中数量增多,通过提高脾脏免疫反应性促进胃溃疡愈合;溃疡期间Mφ数量增多,提示Mφ在溃疡形成和自愈机制中起一定的作用。
[Abstract]:Objective to investigate the changes and effects of splenic dendritic cells (DCG) and macrophages M 蠁 (M 蠁) and T cells in rats with experimental gastric ulcer. The changes of DCM 蠁 and T cells in the spleen of 5 rats in the normal group, 28 in the saline group and 35 in the ulcer group were detected by immunohistochemistry. Compared with the normal group, the number of DCM 蠁 and T cells in the saline group was increased, and the number density of DC in the 10 d group of ulcers increased gradually, and decreased slightly after 14 days. On the 6th day, there was a significant difference between the two groups on the 14th day (P 0.05, P 0.05, P 0.01), and the density of M 蠁 in the ulcer group on the 2nd day, 4th day, in the ulcer group, increased gradually, and decreased gradually at the 14th day. The values of T cells in each group were higher than those in the saline group and the normal group, and the number density of T cells in the ulcer group increased continuously for 2 days and decreased gradually after 10 days. Conclusion the number of DC and T cells in the spleen increased during ulceration, and the number of M 蠁 increased during ulceration by enhancing the immune reactivity of spleen. These results suggest that M 蠁 may play a role in the formation and self healing of ulcers.
【作者单位】：河北北方学院;张家口学院;
【基金】：张家口市科技攻关计划(12110065G-1) 河北北方学院自然科学研究计划项目(No.2001022)
【分类号】：R573.1

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