人类原肌球蛋白亚型5在溃疡性结肠炎中的表达及临床意义

发布时间：2018-02-24 10:28

本文关键词： 抗人类原肌球蛋白亚型5（hTM5）抗中性粒细胞胞浆抗体（ANCA）溃疡性结肠炎（UC）　出处：《山西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的通过检测UC患者血清hTM5、ANCA与肠粘膜hTM5的表达，探讨hTM5在UC中的临床意义。材料和方法收集2012年4月至2013年6月山西医科大学第一临床医院消化科门诊和住院UC患者60例的血清、肠粘膜标本及临床资料，其中UC中重度组30例，UC轻度组30例，诊断符合2012广州会议“对我国IBD诊断治疗规范的共识意见”[1]。采用ELISA方法检测外周血中原肌球蛋白亚型5抗体含量，对照组为健康志愿者30例。结肠粘膜组织行免疫组织化学方法检测肠粘膜原肌球蛋白亚型5阳性细胞数，对照组来自结肠癌行外科手术切除的正常结肠断端。此外,还应用间接免疫荧光法检测UC患者60例及健康对照组30例的血清中抗中性粒细胞胞浆抗体的阳性率。结果（1）免疫组织化学法检测结果：中重度UC患者、轻度UC患者、健康对照人群结肠上皮细胞中原肌球蛋白亚型5阳性表达率差异有统计学意义(P＜0.05)。（2）酶联免疫吸附法检测结果：中重度UC患者、轻度UC患者、健康对照人群血清中原肌球蛋白亚型5抗体浓度差异有统计学意义(P＜0.05)。（3）间接免疫荧光法检测结果：UC患者血清ANCA阳性率明显高于健康对照组，差异有统计学意义（p＜0.05）。（4）血清hTM5浓度和ANCA阳性率做Spearman等级相关分析，发现血清hTM5浓度与ANCA阳性率呈正相关（r=0.428.p=0.000）。结论（1）UC患者结肠粘膜及血清中原肌球蛋白亚型5抗体的表达程度与UC病情轻重程度均呈正相关，提示原肌球蛋白亚型5影响UC的发病及严重程度。（2）联合检测血清原肌球蛋白亚型5和抗中性粒细胞胞浆抗体有助于对UC的诊断与严重程度的判断。
[Abstract]:Purpose. To investigate the clinical significance of hTM5 in UC by detecting the expression of hTM5 ANCA and intestinal mucosal hTM5 in patients with UC. Materials and methods. From April 2012 to June 2013, 60 patients with UC were collected from the Department of Digestive Medicine of the first Clinical Hospital of Shanxi Medical University, including 30 cases of moderate and severe UC and 30 cases of mild UC. The diagnosis was in accordance with the "consensus opinion on the criteria for diagnosis and treatment of IBD in China" [1]. ELISA method was used to detect the level of proto-myosin subtype 5 antibody in peripheral blood. The number of positive cells of myosin subtype 5 in intestinal mucosa was detected by immunohistochemical method in 30 healthy volunteers, and the control group came from the normal cut end of colon cancer surgically resected. The positive rates of anti-neutrophil cytoplasmic antibodies in 60 UC patients and 30 healthy controls were also detected by indirect immunofluorescence assay. Results. Immunohistochemical results: moderate and severe UC patients, mild UC patients, The positive rate of myosin subtype 5 in colonic epithelial cells of healthy controls was significantly different (P < 0.05). The results of enzyme-linked immunosorbent assay (Elisa) were as follows: moderate and severe UC patients, mild UC patients, and mild UC patients. The results of indirect immunofluorescence assay showed that the positive rate of serum ANCA in patients with 20% UC was significantly higher than that in healthy controls. The difference was statistically significant (P < 0.05) the serum hTM5 concentration and the positive rate of ANCA were analyzed by Spearman grade correlation analysis. It was found that the serum hTM5 concentration was positively correlated with the positive rate of ANCA. Conclusion. The expression of myosin subtype 5 antibody in colonic mucosa and serum was positively correlated with the severity of UC, suggesting that myosin subtype 5 affected the incidence and severity of UC. (2) combined detection of promyosin subtype 5 and anti-neutrophil cytoplasmic antibody is helpful to the diagnosis and severity of UC.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R574.62

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