小鼠胚胎干细胞不同分化阶段UGT1a1、UGT1a6和mGST1的表达特征

发布时间：2018-02-26 22:46

本文关键词： 胚胎干细胞肝细胞组织工程干细胞脐带脐血干细胞小鼠胚胎干细胞肝组织定向分化分化阶段 UGTa UGTa mG ST 表达特征胚层结构　出处：《中国组织工程研究》2016年28期 　论文类型：期刊论文

【摘要】：背景:目前对于小鼠胚胎干细胞不同分化阶段的尿普二磷酸葡萄糖醛酞转移酶1a1、尿普二磷酸葡萄糖醛酞转移酶1a6和微粒体谷肤甘肤S-转移酶1表达特征缺乏研究,报道较少。目的:观察小鼠胚胎干细胞不同分化阶段普二磷酸葡萄糖醛酞转移酶1a1、尿普二磷酸葡萄糖醛酞转移酶1a6和微粒体谷肤甘肤S-转移酶1的表达特征。方法:分离培养Wistar大鼠胚胎成纤维细胞,制备饲养层细胞。将胚胎干细胞接种于饲养层细胞上,诱导胚胎干细胞分化为肝细胞,采用Western blot检测尿普二磷酸葡萄糖醛酞转移酶1a1、尿普二磷酸葡萄糖醛酞转移酶1a6和微粒体谷肤甘肤S-转移酶1表达特征,色谱法测定计算微粒体谷肤甘肤S-转移酶1催化活性。结果与结论:在胚胎干细胞在分化为肝细胞过程中,尿普二磷酸葡萄糖醛酞转移酶1a1呈现上升趋势;尿普二磷酸葡萄糖醛酞转移酶1a6在分化起初并未发现其表达,而在分化第18天后表达相对较高;微粒体谷肤甘肤S-转移酶1在胚胎干细胞分化为干细胞整个过程中均具有较高的表达,但表达丰度均低于成年小鼠干细胞。干细胞分化第18天,肝组织微粒存在微粒体谷肤甘肤S-转移酶1催化活性为7.65μmol/(min·g)。结果表明,胚胎干细胞分化为肝细胞过程中,尿普二磷酸葡萄糖醛酞转移酶1a1相对比较稳定,尿普二磷酸葡萄糖醛酞转移酶1a6在分化不同过程中呈现出上升趋势,而微粒体谷肤甘肤S-转移酶1在胚胎干细胞分化为肝细胞过程中表达甚微。
[Abstract]:Background: at present, there is a lack of research on the expression characteristics of UDP glucosaldehyde-phthaletransferase 1a1, UDP glucosaldehyde-phthaletransferase 1a6 and microsomal gluconectin S-transferase 1 in different differentiation stages of mouse embryonic stem cells. Objective: to observe the expression characteristics of Glucosyltransferase 1a1, UDP 1a6 and microsomal glucomannin S-transferase 1 at different differentiation stages of mouse embryonic stem cells. Methods: Wistar rat embryonic fibroblasts were isolated and cultured. To prepare feeder layer cells. Embryonic stem cells were inoculated on feeder layer cells to induce embryonic stem cells to differentiate into hepatocytes. Western blot was used to detect the expression of urinary glucosylaldehyde-transferase (1a1), glucosyltransferase (1a6) and microsomal glucosyltransferase 1 (S-transferase 1). Results and conclusion: in the process of differentiation of embryonic stem cells into hepatocytes, urine phosphoglucomaldehyde-phthaletransferase 1a1 showed an increasing trend. At the beginning of differentiation, the expression of UDP Glucotransferase 1a6 was not found, but the expression was relatively high at the 18th day after differentiation. Microsomal SS-transferase 1 was highly expressed during the differentiation of embryonic stem cells into stem cells, but the expression abundance was lower than that of adult mouse stem cells. The catalytic activity of microsomal glutenin S-transferase 1 in liver tissue was 7.65 渭 mol/(min 路gr. The results showed that the urine gluconaldehyde diphosphate phthaletransferase 1a1 was relatively stable during the differentiation of embryonic stem cells into hepatocytes. UDP glucosaldehyde-phthaletransferase 1a6 showed an upward trend in different differentiation processes, while microsomal glucomannin S-transferase 1 expressed very little in the differentiation of embryonic stem cells into hepatocytes.
【作者单位】：南通大学医学院;
【分类号】：R575

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