不同造模方法的胃肠动力障碍动物模型比较

发布时间：2018-02-28 03:39

本文关键词： 胃肠动力障碍动物模型胃内残留率小肠推进率　出处：《中国老年学杂志》2015年16期 　论文类型：期刊论文

【摘要】：目的比较不同方法建立的胃肠动力障碍动物模型,为胃肠动力障碍疾病研究中动物模型的建立、选择与实际应用提供实验依据。方法将80只昆明种小鼠,分为8组,每组10只。A组空白对照组;B组腹腔注射生理盐水组;C组生理盐水灌胃组;D组甘草煎剂造模组;E组肢体缺血-再灌注造模组;F组冰水刺激造模组;G组阿托品造模组;H精氨酸造模组。造模结束后,测定实验小鼠胃内残留率和小肠推进率,比较各组胃肠动力障碍的发生程度。结果各组模型小鼠胃内残留率分别为:A组43.5%,B组42.6%,C组42.1%,D组70.7%,E组57.2%,F组56.4%,G组59.5%,H组66.2%;各组模型小鼠小肠推进率分别为A组90.7%,B组89.1%,C组89.3%,D组62.2%,E组75.9%,F组71.5%,G组68.1%,H组72.1%。结论甘草煎剂造模组出现胃肠动力障碍最明显,造模可靠。
[Abstract]:Objective to compare the animal models of gastrointestinal motility disorder established by different methods, and to provide experimental basis for the establishment, selection and practical application of animal models in the study of gastrointestinal motility disorders. Methods 80 Kunming mice were divided into 8 groups. Each group of 10 rats in group A, blank control group, group B, intraperitoneal injection of normal saline group, group C, group C, normal saline group, group D, licorice decoction group, group E, group E, limb ischemia-reperfusion model group, group F, ice water stimulation model group, group G, group G, group Atropine model group, H arginine model group. After modeling, The gastric residual rate and small intestinal propulsion rate were measured in experimental mice. Results the gastric residual rate of each group of model mice was 43.5 and 42.6 in group B respectively. The gastric residual rate in group D was 70.7 and that in group E was 57.20.The small bowel propulsion rate in each group was 89.3D in group A and 89.1C in group B, and in group E 57.22 and in group F 59.5H.The intestinal propulsive rate in each group was 90.7 in group A and 89.1g in group C, and 89.3D in group C was higher than that in group B and in group C, respectively, in group A and group B, in group B, in group B, in group B, in group B, in group B, in group B. Group 62.2, group E 75.9 and group F 71.5 and group G 68.1 and group H 72.1. Conclusion Gastrointestinal motility disorder is most obvious in the model group of Glycyrrhiza uralensis decoction. The model is reliable.
【作者单位】：广东药学院基础学院;
【基金】：广东省科技计划项目(2013B021800270)
【分类号】：R-332;R57

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