白藜芦醇对小鼠非酒精性脂肪性肝炎干预作用

发布时间：2018-03-01 20:01

本文关键词： 白藜芦醇(RSV) CBL/J小鼠非酒精性脂肪性肝炎(NASH) 自噬　出处：《中国公共卫生》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨自噬通路在白藜芦醇(RSV)减轻蛋氨酸-胆碱缺乏(MCD)饮食诱导的非酒精性脂肪性肝炎(NASH)中的作用及机制。方法雄性C57BL/6J小鼠40只,随机分为对照组、模型组、白藜芦醇低、高剂量组(100、250 mg/kg)(n=10);对照组和模型组小鼠分别喂饲基础饲料和MCD饲料,白藜芦醇组在喂饲MCD饲料同时给予RSV灌胃,4周后测定小鼠体重、肝重、血清中谷丙转氨酶(ALT)含量,分离小鼠肝脏,测定甘油三酯(TG)和丙二醛含量,苏木素-伊红染色观察肝组织形态学变化,油红O染色观察脂肪变性情况,Western blot检测自噬标志蛋白LC3及各通路蛋白的表达量。结果与对照组比较,模型组小鼠血清ALT和肝组织TG含量[分别为(144.17±0.32)U/L和(112.77±0.88)mg/g]明显升高(P0.01),肝脏LC3Ⅱ和腺苷酸活化蛋白激酶(AMPK)表达水平[分别为(1.27±0.01)、(1.81±0.09)]升高(P0.05);与模型组比较,高剂量白藜芦醇组小鼠血清ALT含量、肝组织TG含量[分别为(83.16±0.40)U/L、(40.75±0.19)mg/g]明显降低(P0.01),LC3Ⅱ、AMPK蛋白表达水平[分别为(2.25±0.14)、(3.01±0.16)]明显升高(P0.01);蛋白激酶B(Akt)和哺乳动物雷帕霉素靶蛋白(m TOR)表达水平无明显变化。结论白藜芦醇可以改善MCD饮食诱导的NASH,其机制可能与激活自噬AMPK途径有关。
[Abstract]:Objective to investigate the autophagy pathway in resveratrol (RSV) reduced methionine choline deficient (MCD) diet of nonalcoholic steatohepatitis (NASH) induced by the action and mechanism. Methods 40 male C57BL/6J mice were randomly divided into control group, model group, resveratrol low, high dose group (100250 mg/kg) (n=10); control group and model group mice were fed with basic feed and MCD, resveratrol group were fed with MCD diet and given RSV intragastric administration, the mice were detected after 4 weeks, liver weight, serum alanine aminotransferase (ALT) were isolated from mouse liver, determination of triglycerides (TG) and the content of MDA, hematoxylin eosin staining to observe the morphological changes of liver tissue, oil red O staining to observe the fatty degeneration, Western blot detection of autophagy marker protein expression and the LC3 pathway protein. Results compared with the control group, the TG content in serum of mice liver tissue and ALT model respectively. (144.17 + 0.32) U/L and (112.77 + 0.88) mg/g] increased significantly (P0.01), and LC3 II liver adenylate activated protein kinase (AMPK) expression level respectively [(1.27 + 0.01), (1.81 + 0.09)] increased (P0.05); compared with the model group, high dose of resveratrol treated mice the content of ALT in serum, the content of TG in liver tissue were [(83.16 + 0.40) U/L, (40.75 + 0.19) mg/g] decreased significantly (P0.01), LC3 II, AMPK protein expression level respectively [(2.25 + 0.14), (3.01 + 0.16)] significantly increased (P0.01); protein kinase B (Akt) and mammalian target of rapamycin (m TOR) level had no obvious change. Conclusion resveratrol improves MCD diet induced NASH, the mechanism may be associated with the activation of autophagy pathway of AMPK.

【作者单位】：中山大学公共卫生学院;广东省疾病预防控制中心广东省公共卫生研究院;
【基金】：国家自然科学基金(81200295)
【分类号】：R575.5

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