头孢克肟诱导肠道菌群紊乱动物模型的建立

发布时间：2018-03-04 16:44

本文选题：头孢克肟　切入点：肠道菌群　出处：《食品工业科技》2017年05期 　论文类型：期刊论文

【摘要】：目的:构建头孢克肟诱导的小鼠肠道菌群紊乱模型。方法:将40只体重18~22 g的SPF级BALB/c雌性小鼠随机分为正常对照组、头孢克肟低剂量组、中剂量组、高剂量组,每组10只。灌胃剂量分别为187.75 mg/(kg·d),375.50 mg/(kg·d),563.25 mg/(kg·d),正常对照组灌胃相同剂量的蒸馏水。连续灌胃7 d后颈椎脱臼处死并收集盲肠内容物,采用传统培养结合聚合酶链式反应-变性梯度凝胶电泳技术(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)观察和分析头孢克肟对小鼠肠道菌群的影响。结果:高剂量组小鼠粪便中乳酸杆菌数量在所选稀释度下降至30CFU以下,而肠球菌数量上升至1.26×105CFU,大肠杆菌数量上升至1.86×106CFU。与空白对照组相比,模型组小鼠肠道菌群总体多样性和细菌种类显著降低。结论:中剂量和高剂量头孢克肟均能不同程度的诱导小鼠肠道菌群紊乱模型,并且肠道菌群紊乱程度随灌胃剂量的增加而加重,模型成功建立。
[Abstract]:Objective: to establish a model of intestinal flora disorder induced by cefixime in mice. Methods: forty SPF grade BALB/c female mice weighing 1822 g were randomly divided into normal control group, cefixime low dose group, middle dose group and high dose group. 10 rats in each group were perfused at a dose of 187.75 mg/(kg 路danlun 375.50 mg/(kg 路danlun 563.25 mg/(kg 路dl, respectively. The normal control group was given distilled water of the same dose. The cervical vertebrae dislocated and the cecum contents were collected after 7 days of continuous gavage. Traditional culture combined with polymerase chain reaction-denaturing gradient gel electrophoresisPCR-DGGE was used to observe and analyze the effect of cefixime on the intestinal flora of mice. Results: the number of lactobacillus in feces of high dose group mice. When the selected dilution drops below 30 CFU, The number of Enterococcus increased to 1.26 脳 10 5 CFU and the number of Escherichia coli to 1.86 脳 10 6 CFU. Conclusion: both middle and high dose cefixime can induce intestinal flora disturbance in mice. The disturbance degree of intestinal flora was aggravated with the increase of gastric administration dose, and the model was established successfully.
【作者单位】：天津科技大学食品工程与生物技术学院;
【基金】：国家科技支撑计划项目(2011BAD23B02)
【分类号】：R-332;R574

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