IL-2联合肝细胞生长因子体外诱导大鼠骨髓间充质干细胞向肝细胞分化的效果观察

发布时间：2018-03-05 08:34

本文选题：骨髓间充质干细胞　切入点：白细胞介素　出处：《山东医药》2017年34期 　论文类型：期刊论文

【摘要】：目的观察白细胞介素2(IL-2)联合肝细胞生长因子(HGF)体外诱导大鼠骨髓间充质干细胞(BMSCs)分化为肝细胞的效果。方法将大鼠P3代BMSCs随机分为5组,M0组不处理,M1组加20μg/L HGF,M2、M3、M4组分别加5、10、20μg/L IL-2和20μg/L HGF。于第7、14、21天倒置相差显微镜下观察各组细胞形态变化,用免疫细胞化学方法检测细胞中的白蛋白及甲胎蛋白。结果 M0组细胞呈长梭形,在诱导过程中细胞形态没有发生改变;而M1~M4组由长梭形细胞逐渐向三角形、类圆形转化,随着时间延长逐渐增多,类似肝细胞。M0组细胞各时点均未检测到甲胎蛋白和白蛋白;M1~M4组随诱导时间延长甲胎蛋白表达逐渐减少(P均0.05)而白蛋白表达逐渐增加(P均0.05),且同一时间点甲胎蛋白、白蛋白表达M1~M4组逐渐增加(P均0.05)。结论IL-2联合HGF可体外诱导大鼠BMSCs分化为肝细胞,IL-2浓度越高诱导生成的肝细胞越多。
[Abstract]:Objective to observe the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into hepatocytes induced by interleukin-2 (IL-2) combined with hepatocyte growth factor (HGF) in vitro. Methods the P3 generation of rat BMSCs was randomly divided into 5 groups without treatment of M0 group and 20 渭 g / L HGFM2M2M3M4 group. The morphologic changes of cells in each group were observed under inverted phase-contrast microscope on day 714 ~ (-1), respectively, and 20 渭 g / L IL-2 and 20 渭 g / L HGF were added to 5 ~ 10 渭 g / L and 20 渭 g / L HGF, respectively. Results the cells in M0 group were fusiform, but the morphology of M1M4 cells was not changed during induction, while in M1M4 group, the cells were transformed from long fusiform cells to triangles and round cells. As time goes on, The expression of Alpha-fetoprotein and Albumin M1-M4 decreased gradually with the prolongation of induction time (P < 0.05), but the expression of albumin increased gradually (P < 0.05), and at the same time point, the expression of Alpha-fetoprotein was increased, and at the same time point, the expression of Alpha-fetoprotein was increased. Conclusion IL-2 combined with HGF can induce rat BMSCs to differentiate into hepatocytes and produce more hepatocytes with higher concentration of IL-2.
【作者单位】：西南医科大学附属医院;
【基金】：四川省卫生厅科研项目(110358)
【分类号】：R575.3

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