深圳市无偿献血者隐匿性HBV感染发展预后以及性传播的研究

发布时间：2018-03-05 12:21

本文选题：献血者　切入点：隐匿性乙型肝炎病毒感染　出处：《中国输血杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的研究本地区无偿献血者隐匿性HBV感染(OBI)发展预后以及性传播的可能。方法追踪随访25名来自2010-2012年确认为OBI的无偿献血者及其配偶,采用化学发光方法检测HBs Ag,常规核酸检测方法(NAT)及Nested-PCR方法扩增BCP/PC、S基因确认HBV DNA的存在;实时荧光定量检测方法(QPCR)定量乙肝病毒载量。结果从25名OBI献血者,成功追踪回访15名及6/15名献血者配偶,15名OBI献血者男女比例为8∶7,病毒载量范围在(10-121.8)IU/m L(中位数14.3 IU/m L),9/15例为抗-HBc阳性。在2-4年后的随访结果,15名OBI献血者有4名(26.7%)转为显性HBV感染(HBs Ag+/HBV DNA+),7名(46.6%)献血者HBV转阴(HBs Ag-/HBV DNA-),4名(26.7%)仍呈OBI持续感染状态(HBs Ag-/HBV DNA+)。OBI转为HBV的献血者的病毒载量显著高于原来OBI感染状态(P0.05),而仍维持OBI感染献血者的病毒载量与2-4年前相比没有统计学差异(P0.05)。6名OBI献血者的配偶,均为没有感染HBV(HBs Ag-/HBV DNA-)。结论感染OBI献血者存在自然清除病毒及转为显性HBV感染的发展预后,HBV感染者的起源是否与OBI有关,有待进一步探索。
[Abstract]:Objective to study the prognosis of occult HBV infection and the possibility of sexual transmission among unpaid blood donors in this area. Methods 25 unpaid blood donors and their spouses who were confirmed as OBI from 2010 to 2012 were followed up. The presence of HBV DNA was confirmed by chemiluminescence assay (CL), routine nucleic acid detection (Nat) and Nested-PCR amplification, and real-time fluorescence quantitative analysis (RFT) was used to quantify HBV load. The male / female ratio of 15 successful follow-up visits and 15 OBI donors was 8: 7, and the viral load ranged from 10 to 121.8 渭 m L (median 14.3 IU/m L / 9 / 15 cases were anti-HBc positive. 2 to 4 years later, 4 out of 15 OBI blood donors were found to be positive for anti-HBc). HBs Ag / HBV DNA / 7 patients with dominant HBV infection (46.6)) Blood donors with HBs Ag-/HBV DNA were still in OBI persistent infection status. HBs Ag-/HBV DNA. Obi blood donors with HBV were significantly higher than those with OBI infection status P0.055whereas those who still had OBI infection had higher viral load than those who had been infected with HBs / HBV DNA. There was no significant difference in viral load between two and four years ago among the spouses of P0.05.6 OBI donors. Conclusion the development and prognosis of HBV(HBs Ag-/HBV DNA-infection in infected OBI blood donors may be related to the origin of OBI infection, which should be further explored.
【作者单位】：深圳市血液中心;
【基金】：深圳市科技计划项目(201302153)
【分类号】：R512.62

【参考文献】