复方甘草酸苷对自身免疫性肝炎患者肝脏单核巨噬细胞吞噬功能的影响及机制

发布时间：2018-03-08 03:27

本文选题：自身免疫性肝炎　切入点：复方甘草酸苷　出处：《山东医药》2017年24期 　论文类型：期刊论文

【摘要】：目的探讨复方甘草酸苷对自身免疫性肝炎(AIH)患者肝脏单核巨噬细胞吞噬功能的影响及机制。方法选择AIH患者30例,分离并培养肝脏单核巨噬细胞,取培养2天的细胞随机分为两组,观察组加入终浓度为1 mg/m L的甘草酸苷,对照组加入等量缓冲液,处理30 min。采用荧光显微镜观察细胞吞噬能力,计算吞噬率及吞噬指数;采用流式细胞仪检测细胞表面抗原提呈分子CD80、CD40、人类白细胞抗原DR(HLA-DR)表达。结果观察组细胞吞噬率为(49.80±1.36)%、吞噬指数为80.36±4.70,对照组分别为(26.38±3.25)%、32.38±1.79,观察组均高于对照组(P均0.01)。观察组细胞表面CD40、CD80、HLA-DR相对表达量分别为0.768±0.031、0.768±0.031、0.937±0.062,对照组分别为0.452±0.023、0.452±0.023、0.548±0.032,观察组均高于对照组(P均0.01)。结论复方甘草酸苷可提高AIH患者肝脏单核巨噬细胞的吞噬功能;增加单核巨噬细胞抗原呈递能力可能是其作用机制。
[Abstract]:Objective to investigate the effect and mechanism of compound glycyrrhizin on the phagocytosis of liver mononuclear macrophages in patients with autoimmune hepatitis. Methods 30 patients with AIH were selected to isolate and culture mononuclear macrophages. The cells cultured for 2 days were randomly divided into two groups. In the observation group, the final concentration of glycyrrhizin was 1 mg/m / L, and the control group was treated with the same amount of buffer solution for 30 minutes. The phagocytic ability of the cells was observed by fluorescence microscope, and the phagocytosis rate and phagocytic index were calculated. Flow cytometry was used to detect the expression of CD80 CD40 and HLA-DRN. Results the phagocytic rate of cells in the observation group was 49.80 卤1.36 and the phagocytic index was 80.36 卤4.70, respectively, and that in the control group was 32.38 卤1.79, which was higher than that in the control group (P 0.01). The relative expression of HLA-DR was 0.768 卤0.031, 0.768 卤0.031, 0.768 卤0.031, 0.937 卤0.062, respectively, and 0.452 卤0.023, 0.452 卤0.023, 0.452 卤0.023, 0.548 卤0.032 in the control group, which was higher than that in the control group (P < 0.01). Conclusion compound glycyrrhizin can improve the phagocytic function of mononuclear macrophages in AIH patients. Increasing the antigen presentation capacity of mononuclear macrophages may be its mechanism.
【作者单位】：天津市泰达医院;天津医科大学;枣庄矿业集团中心医院;天津医科大学总医院;
【基金】：天津市滨海新区卫生局科技项目(2013BWKY017)
【分类号】：R575.1

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