布加综合征患者腔房转流术后血清MAPK相关蛋白的表达及意义

发布时间：2018-03-08 15:20

本文选题：腔房转流术　切入点：布加综合征　出处：《中国现代医学杂志》2017年17期 　论文类型：期刊论文

【摘要】：目的观察布加综合征(BCS)患者腔房转流术后血清丝裂原活化蛋白激酶(MAPK)相关蛋白的表达。方法选取腔房转流术后BCS患者(观察组)和健康志愿者(对照组),各40例。采用Western blot检测术前和术后3 d及1个月血清MAPK相关蛋白表达。结果 (1)观察组术前和术后各时间点血清胞外信号调节激酶1/2(ERK1/2)、p38、c-Jun氨基末端激酶及胞外信号调节激酶5(ERK5)的表达水平与对照组比较,差异无统计学意义(P0.05),且观察组术前表达水平与术后3 d及术后1个月比较,差异无统计学意义(P0.05);(2)观察组术前和术后各时间P-ERK1/2、P-p38及磷酸化c-Jun氨基末端激酶(P-JNK)表达水平均高于对照组,P-ERK5低于对照组;(3)观察组术后3 d的P-ERK1/2、P-p38及P-JNK表达水平较术前上升,而P-ERK5较术前下降。结论腔房转流术可上调BCS患者血清P-ERK1/2、P-p38及P-JNK的表达水平,下调血清P-ERK5的表达水平,而对各总蛋白的表达无调控作用。
[Abstract]:Objective to observe the expression of mitogen-activated protein kinase (MAPK) related proteins in patients with Budd-Chiari syndrome (BCS) after cavities atrial bypass (CABG). Methods 40 patients with BCS and 40 healthy volunteers (control group) after CABG were selected as control group (n = 40) and control group (n = 40). Western blot was used to detect the expression of serum MAPK related protein before and after 3 days and 1 month after operation. Results: in the observation group, serum extracellular signal regulated kinase 1 / 2 ERK1 / 2 was measured before and 3 days after operation, and the expression of c-Jun amino terminal kinase and extracellular signal regulated kinase were measured. The expression level of ERK5) was compared with that of the control group. There was no significant difference between the two groups (P 0.05), and the expression level before operation in the observation group was compared with that on the 3rd day after operation and 1 month after operation. The expression levels of P-ERK1 / 2p38 and phosphorylated c-Jun amino-terminal kinase (P-JNK5) in the observation group were significantly higher than those in the control group 3 days after operation. The expression levels of P-ERK1 / 2P-p38 and P-JNK in the observation group were higher than those in the control group 3 days after operation. Conclusion the expression of P-ERK1 / 2 p38 and P-JNK in serum of patients with BCS can be upregulated, and the expression of P-ERK5 is down-regulated, but the expression of total protein is not regulated.
【作者单位】：郑州大学人民医院肝胆外科;
【基金】：河南省医学科技攻关计划重点攻关项目(No:200902006)
【分类号】：R575

【相似文献】