溃疡性结肠炎患者DR4和DR5基因单核苷酸多态性分析

发布时间：2018-03-10 06:32

本文选题：溃疡性结肠炎　切入点：TRAIL　出处：《实用医学杂志》2015年22期 　论文类型：期刊论文

【摘要】：目的:探讨死亡受体(DR)4和DR5基因单核苷酸多态性(SNP)与溃疡性结肠炎(UC)的关系。方法:选取163例UC患者和252例对照者,采用直接测序法检测DR4(rs20575)和DR5(rs1047266)的基因多态性。结果:与对照组相比,UC组中DR4(rs20575)突变等位基因G和基因型CG+GG的频率显著升高(6.75%vs.1.39%,P0.001,OR=5.138;8.59%vs.2.78%,P=0.008,OR=3.289)。分层分析发现重度UC患者中DR4(rs20575)突变基因型CG+GG的频率显著高于轻中度患者(20.83%vs.6.47%,P=0.020,OR=3.801);广泛结肠炎患者中DR4(rs20575)突变等位基因G的频率较远端结肠炎显著降低(2.34%vs.9.60%,P=0.011,OR=0.226)。DR5(rs1047266)的突变等位基因和基因型频率在UC组和对照组之间比较差异无统计学意义(均P0.05)。结论:DR4(rs20575)基因多态性与UC易感性密切相关,并且可能影响UC的疾病严重程度和病变部位。DR5(rs1047266)基因多态性与UC易感性无关。
[Abstract]:Objective: to investigate the relationship between single nucleotide polymorphisms (SNPs) of death receptor DR4 and DR5 gene and ulcerative colitis (UC). Methods: 163 UC patients and 252 controls were selected. Direct sequencing method was used to detect the polymorphism of DR4 rs20575) and DR5 rs1047266). Results: compared with the control group, the frequency of allele G and genotype CG GG of DR4 rs20575) was significantly increased in patients with severe UC. The mutation of DR4rs20575 was found in severe UC patients by stratified analysis of DR4rs20575). The frequency of type type CG GG was significantly higher than that of mild to moderate patients (20.83vs.6.470.2020OR3.801); in patients with extensive colitis, the frequency of DR4rs20575) mutation allele G was significantly lower than that of distal colitis patients (2.34vs.9.60th P0.011OR0.226h.DR5rs1047266)) and the frequency of mutation allele and genotype was compared between UC group and control group. There was no significant difference (P 0.05). Conclusion the gene polymorphism of 1% DR4 rs20575 is closely related to the susceptibility to UC. Moreover, the polymorphism of DR5 rs1047266 gene was not associated with UC susceptibility.
【作者单位】：浙江省温州市中心医院浙江省温州医科大学附属定理医院;
【基金】：2013年省卫生厅一般项目(编号:2013KYB251)
【分类号】：R574.62

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