人羊膜间充质干细胞移植后慢性酒精性肝损伤大鼠血生化指标含量的变化

发布时间：2018-03-11 13:20

本文选题：人羊膜间充质干细胞　切入点：大鼠　出处：《中国比较医学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的有研究表明人羊膜间充质干细胞(human amniotic mesenchymal stem cells,h AMSCs)具有多种分化和增殖功能,其可以诱导分化成肝样细胞,从而对肝损伤有一定的修复作用。方法体外复苏培养人羊膜间充质干细胞,66只健康雌性Wistar大鼠,随机取22只,不做任何处理为正常对照组,余44只采用白酒灌胃30 d的方式建立大鼠慢性酒精性肝损伤模型,建模后将其随机分为模型组(尾静脉注射1 m L PBS),h AMSCs组(经尾静脉注入1 m L h AMSCs(2×106个),每组各22只。于移植后4周,采用全自动生化分析仪分别检测各组大鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、血清总胆红素(TBIL)、血清白蛋白(ALB)、血清总蛋白(Tp);同时检测检测各组大鼠肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)、以及丙二醛(MDA)、IL-4含量变化;免疫荧光显微镜观察PKH-26标记的h AMSCs分布情况,TUNEL法检测各组肝细胞凋亡情况。结果各组大鼠血生化检测结果显示,与正常对照组比较,模型组中ALT、AST、TBIL、Tp含量均显著升高,而与模型组比较,h AMSCs移植组中ALT、AST、TBIL、Tp含量均显著下降(P0.05),与正常对照组比较,模型组中ALB含量明显下降,而与模型组比较,h AMSCs移植组中ALB含量明显升高(P0.05);与模型组比较,h AMSCs移植组SOD、GSH-PX、CAT含量均显著增高,MDA、IL-4含量均显著下降,与正常对照组比较,h AMSCs移植组SOD、GSH-PX、CAT含量均降低,MDA、IL-4含量均升高(P0.05);移植组可见移植的PKH-26标记阳性的h AMSCs细胞分布于肝脏组织内,其余各组未见此类细胞分布(P0.05);TUNEL法检测各组肝细胞凋亡可见凋亡细胞数,模型组最多(34.27±5.71),h AMSCs移植组次之(18.42±3.95),正常对照组未见凋亡细胞。结论人羊膜间充质干细胞移植能够改善大鼠肝硬化的血生化指标水平,h AMSCs移植后可以减轻慢性酒精性肝损伤大鼠的肝脏细胞凋亡。
[Abstract]:The purpose of research has shown that human amniotic mesenchymal stem cells (human amniotic mesenchymal stem cells, H AMSCs) with a variety of differentiation and proliferation, which can be differentiated into hepatocyte like cells, so as to repair the damaged liver function. Human amniotic mesenchymal stem cell culture method in vitro recovery, 66 healthy female Wistar in rats, 22 rats were randomly selected, without any treatment as normal control group, more than 44 only by liquor gavage 30 d to establish rat model of chronic alcoholic liver injury model, after modeling were randomly divided into model group (intravenous injection of 1 m L PBS), H group (AMSCs injection 1 m L h AMSCs via the tail vein (2 x 106), 22 rats in each group. In 4 weeks after transplantation, using automatic biochemical analyzer to detect the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum total bilirubin (TBIL), serum albumin (ALB serum). The total protein (Tp); at the same time, detection of liver superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), and malondialdehyde (MDA), the change of the content of IL-4 h AMSCs; the distribution of PKH-26 labeled immunofluorescence microscopy, testing the apoptosis of liver cells were TUNEL method. Results the rats blood biochemical test results showed that compared with normal control group, model group, ALT, AST, TBIL, Tp were significantly increased compared with the model group, H transplantation group AMSCs ALT, AST, TBIL, Tp were significantly decreased (P0.05), compared with the normal control group and the content of ALB was decreased in the model group, compared with model group, the content of ALB in the H transplantation group AMSCs increased significantly (P0.05); compared with the model group, H transplantation group, SOD GSH-PX, AMSCs, CAT were significantly increased, MDA and IL-4 content were significantly decreased, compared with normal control group, H transplantation AMSCs Group SOD, GSH-PX, MDA, CAT content decreased, IL-4 content increased (P0.05); transplantation group showed the transplanted PKH-26 labeled h positive AMSCs cell distribution in liver tissue, while there was no such cell distribution (P0.05); to detect the apoptosis of liver cells were apoptotic cells by TUNEL method, model group (the most 34.27 + 5.71), H (18.42 + AMSCs transplantation group 3.95), the normal control group. Conclusion apoptosis of human amniotic mesenchymal stem cell transplantation blood biochemical index level can improve the rat liver cirrhosis, liver cell apoptosis of H AMSCs after transplantation can alleviate the injury of rat chronic alcoholic liver disease.

【作者单位】：武警后勤学院附属医院检验科;武警后勤学院附属医院生殖医学研究中心;大同市第五人民医院重症医学科;
【分类号】：R575

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