人羊膜上皮细胞移植治疗肝纤维化免疫大鼠

发布时间：2018-03-14 23:11

  本文选题：人羊膜上皮细胞　切入点：细胞移植　出处：《中国修复重建外科杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的观察人羊膜上皮细胞(human amniotic epithelial cells,h AECs)在正常免疫大鼠体内存活、迁徙及对大鼠肝纤维化程度的影响,为h AECs应用于临床提供实验依据。方法 10周龄雄性SD大鼠64只,体重220~280 g,随机分为4组,每组16只。A组为肝纤维化+细胞移植(移植细胞量4×106个)组,B组为肝纤维化+生理盐水组,C组为肝纤维化组,D组为空白对照组。移植后2周,取A组大鼠心、肝、脾、肾、肺、脑组织采用DNA-PCR法检测人Alu基因重复序列,免疫组织化学染色观察人白细胞抗原G(hu man leucocyte antigen G,HLA-G)表达,并比较各脏器阳性表达百分比(脾脏为细胞注入器官不纳入统计);取各组肝组织,行HE染色观察并采用肝纤维化组织学半定量计分系统(SSS)行肝纤维化半定量分析,行TGF-β1免疫组织化学染色观察并行免疫组织化学评分(immunohistochemical score,ISH);取各组血清行谷草转氨酶(aspartate transaminase,AS T)、谷丙转氨酶(alanine aminotransferase,ALT)、白蛋白(albumin,ALB)浓度检测,定量分析大鼠肝纤维化程度。结果A组各脏器中人Alu基因重复序列及HLA-G均表达阳性,肝组织中阳性表达百分比显著高于其余组织,比较差异有统计学意义(P0.05)。A、B、C组肝纤维化组织学半定量计分分别为(10.47±3.20)、(13.84±3.46)、(13.85±3.16)分,A组显著低于B、C组(P0.05);B、C组间差异无统计学意义(P0.05)。A、B、C、D组TGF-β1的ISH评分分别为(3.60±1.50)、(5.38±2.60)、(5.50±2.40)、(1.87±1.36)分,A、B、C组显著高于D组,A组显著低于B、C组(P0.05);B、C组间差异无统计学意义(P0.05)。A、B、C组血清AST、ALT浓度显著高于D组,A组显著低于B、C组(P0.05),B、C组间差异无统计学意义(P0.05);ALB浓度显著低于D组,A组显著高于B、C组(P0.05);B、C组间差异无统计学意义(P0.05)。结论 h AECs可在正常免疫大鼠体内存活,可缓解肝纤维化程度并改善血清肝功能指标。
[Abstract]:Objective To observe the effect of human amniotic epithelial cells (human amniotic epithelial cells, H AECs) on the survival of normal immune rats, migration and the influence on the degree of hepatic fibrosis in rats, H AECs provide an experimental basis for clinical application. Methods 10 week old male SD 64 rats, weighing 220~280 g, were randomly divided into 4 groups 16 rats in each group, group.A hepatic fibrosis + cell transplantation (transplantation cells 4 * 106) group, B group of liver fibrosis + saline group, C group for hepatic fibrosis group, D group was the control group. 2 weeks after transplantation, A group rats heart, liver, spleen, kidney, lung, brain tissue by DNA-PCR method for detection of Alu gene repeats, immunohistochemical staining of human leukocyte antigen G (hu man leucocyte antigen G, HLA-G) expression, and compare various organs (spleen cells as the positive expression percentage of injected organs are not included in the statistics); livers tissue, HE staining and the observation of liver fibrosis 缁村寲缁勭粐瀛﹀崐瀹氶噺璁″垎绯荤粺(SSS)琛岃倽绾ょ淮鍖栧崐瀹氶噺鍒嗘瀽,琛孴GF-尾1鍏嶇柅缁勭粐鍖栧�︽煋鑹茶�傚療骞惰�屽厤鐤�缁勭粐鍖栧�﹁瘎鍒�(immunohistochemical score,ISH);鍙栧悇缁勮��娓呰�岃胺鑽夎浆姘ㄩ叄�

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