肿瘤坏死因子-α对大鼠离体灌注肾血管收缩的影响

发布时间：2018-03-18 02:04

本文选题：肿瘤坏死因子-α　切入点：离体灌注肾　出处：《中国老年学杂志》2015年13期 　论文类型：期刊论文

【摘要】：目的观察肿瘤坏死因子(TNF)-α对肾血管收缩的影响及机制。方法 56只大鼠制备离体灌注肾模型,随机分为8组(n=7):A1组:Kreb液灌流;A2组:Kreb液+TNF-α灌流;B1组:Kreb液+Verapamil灌流;B2组:Kreb液+Verapamil+TNF-α灌流;C1组:无钙Kreb液灌流;C2组:无钙Kreb液+TNF-α灌流;D1组:无钙Kreb液+2-APB灌流;D2组:无钙Kreb液+2-APB+TNF-α灌流。各组均在刺激期加内皮素(ET)。灌流结束后,计算肾脏水肿率,HE染色观察肾小球及肾小管形态、结构。结果各组基础灌注压比较无显著差异(P0.05)。A1、A2、B1、B2、C1、C2组ET刺激后,肾灌注压较基础压均明显升高(P0.05);A2、B2、C2组灌注压升高值分别显著高于A1、B1、C1组(P0.01)。D1、D2组ET刺激后,肾灌注压均略升高,但与基础灌注压比较无显著差异(P0.05);两组灌注压升高值比较无显著性差异(P0.05)。8组肾脏的水肿率均低于30%,灌流后肾脏标本切片均未发现明显的器质性损伤,与灌流前相符。结论 TNF-α可能通过上调1,4,5-三磷酸肌醇受体(IP3R)增强ET引起的肾血管收缩。
[Abstract]:Objective to observe the effect and mechanism of TNF- 伪 on renal vasoconstriction in 56 rats. Eight groups were randomly divided into 8 groups: 1% Kreb solution, 2% Kreb solution, A 2 group, 1% Kreb solution, TNF- 伪 perfusion group, 1% Kreb solution, Verapamil solution, 2% Kreb solution, 2% Kreb solution, Verapamil, 2% Kreb solution, 1 group, 2 + calcium free Kreb solution, and C2 group, no calcium Kreb solution, TNF- 伪 perfusion group, no calcium Kreb solution, 2 APB perfusion fluid, D 2 group, D 2 group, D 2 group, respectively. Calcium free Kreb solution 2-APB TNF- 伪 was perfused. The morphology and structure of glomeruli and renal tubules were observed by HE staining. Results there was no significant difference in basal perfusion pressure among the three groups. The renal perfusion pressure was significantly higher than that of the base pressure. After et stimulation, the renal perfusion pressure in the P0.05A2OB2OC2 group was significantly higher than that in the A1-B1OC1 group, and the renal perfusion pressure increased slightly after et stimulation. However, there was no significant difference between the two groups compared with the basic perfusion pressure (P 0.05), and there was no significant difference in the increase of perfusion pressure between the two groups. The edema rate of kidney was lower than that of 30% in the P0.05 group, and no significant organic injury was found in the sections of the renal specimens after perfusion. Conclusion TNF- 伪 may enhance the renal vasoconstriction induced by et by upregulating the receptor IP _ 3R.
【作者单位】：辽宁医学院附属第一医院消化科;辽宁医学院基础学院;
【基金】：辽宁省教育厅高等学校科研计划项目(2009A455) 辽宁省科技厅科技计划项目(2010225034)
【分类号】：R575

【共引文献】