饱和脂肪酸与单不饱和脂肪酸对HepG2细胞自噬和凋亡的影响

发布时间：2018-03-19 08:25

  本文选题：棕榈酸　切入点：油酸　出处：《营养学报》2015年06期 　论文类型：期刊论文

【摘要】：目的比较饱和脂肪酸棕榈酸(palmitic acid,PA)和单不饱和脂肪酸油酸(oleic acid,OA)对HepG2细胞凋亡和自噬的影响。方法 PA和OA处理HepG2细胞,CCK-8检测脂肪酸的细胞毒性,caspase-3活性测定检测脂肪酸对细胞凋亡的影响,油红O染色检测细胞内脂质沉积,酶法检测细胞内甘油三酯的含量,Western blot检测自噬相关蛋白LC3-Ⅱ和凋亡相关蛋白caspase-3的表达,实时荧光定量PCR检测相关基因表达。结果 PA和OA都均同时诱导HepG2细胞凋亡与自噬,但PA的影响明显强于OA;PA诱导HepG2细胞自噬相关基因LC3B和VPS38表达、促进脂肪酸β-氧化的PPARα基因mRNA水平升高,但对细胞内脂肪沉积没有明显作用;而OA则导致HepG2细胞内甘油三酯的水平明显增加,但对上述基因表达没有明显影响。结论饱和脂肪酸PA和不饱和脂肪酸OA均能引起HepG2细胞凋亡和自噬,但PA的作用强于OA。
[Abstract]:Objective to compare the effects of palmitic acido) and monounsaturated fatty acid oleic acidOAA on apoptosis and autophagy of HepG2 cells. Methods HepG2 cells treated with PA and OA were treated with CCK-8 to detect the cytotoxicity of fatty acids and the activity of caspase-3 to detect fat. The effect of acid on apoptosis, Lipid deposition was detected by oil red O staining and triglyceride content was detected by enzyme method. The expression of autophagy associated protein LC3- 鈪，

本文编号：1633443