利用PCR-DGGE分析胆固醇结石和胆汁中细菌群落结构

发布时间：2018-03-19 09:20

本文选题：胆固醇结石　切入点：胆汁　出处：《四川大学学报(自然科学版)》2016年03期 　论文类型：期刊论文

【摘要】：本研究运用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)方法对来自8名胆石病人的8份胆固醇结石和4份胆汁样品中细菌群落结构进行分析和比较研究.DGGE结果显示,胆固醇结石中细菌含有Enterococcus,Pseudomonas等13个属细菌,其中Pseudomonas检出率最高,Klebsiella和Bacillus两个属细菌含量较多;胆固醇结石中6个属细菌在胆汁中也检测到.不同病人之间胆固醇结石细菌群落结构高度相似,但胆汁相似度较低;胆固醇结石中细菌群落结构与胆汁细菌群落结构最高相似度仅为38%,胆汁中细菌种类虽与胆固醇结石有同源性,但细菌群落结构差异较大.因此,导致胆固醇结石形成的细菌种类有一定偏好性,不因人而异,临床上对这些细菌的控制将有利于抑制结石的生成.
[Abstract]:In this study, polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) were used to analyze and compare the bacterial community structure in 8 cholesterol gallstones and 4 bile samples from 8 cholelithiasis patients. There were 13 genera of bacteria in cholesterol stone, including Enterococcus sp. Pseudomonas, among which, the highest detection rate of Pseudomonas was found in Klebsiella and Bacillus. The bacteria of 6 genera in cholesterol stone were also detected in bile. The bacterial community structure of cholesterol stone was highly similar among different patients, but the similarity of bile was lower. The highest similarity between bacterial community structure and bile bacterial community structure in cholesterol stone is only 38. Although the species of bacteria in bile are homologous to cholesterol stone, the structure of bacterial community is quite different. The bacteria that lead to the formation of cholesterol stones have certain preference and do not vary from person to person. The clinical control of these bacteria will help to inhibit the formation of stones.
【作者单位】：四川大学生命科学学院生物资源与生态环境教育部重点实验室;中国人民解放军成都军区总医院;
【基金】：国家自然科学基金(J1103518)
【分类号】：R575.62

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