乙肝表面抗体与年龄的相关研究及影响因素

发布时间：2018-03-20 13:22

本文选题：乙肝表面抗体　切入点：抗体活性　出处：《大连医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：本文通过调查分析发现，乙肝表面抗体随着年龄的增长有一定的降低趋势，这种趋势是否由年龄的原因造成还是由其他的因素所导致，是本文研究的主要目的。方法：实验对象为来自大连医科大学附属第一医院的非肝炎患者，乙肝表面抗体阳性(抗HBs含量10IU/L)、乙肝表面抗原阴性（HBsAg0.05IU/L）、e抗原阴性（HBeAg1IU/L），天门冬氨酸氨基转移酶正常（男15AST40U/L，女15AST35U/L），丙氨酸氨基转移酶正常（男9ALT50U/L，女7ALT40U/L），，选取110例，男55例，女55例。实验分组情况：根据年龄及e抗体，核心抗体阴性或阳性分为四组：单抗体阳性青年组：年龄为24-26岁，e抗体阴性，核心抗体阴性。男10例，女10例。单抗体阳性中年组：年龄为45-50岁，e抗体阴性，核心抗体阴性。男10例，女10例。“2和/或3抗体”阳性青年组：年龄为24-26岁，e抗体或者核心抗体任意一个阳性，或者二者均阳性。男15例，女15例。“2和/或3抗体”阳性中年组：年龄为45-50岁，e抗体或者核心抗体任意一个阳性，或者二者均阳性。男20例，女20例。用罗氏cobas e601全自动电化学发光免疫分析仪，双抗体夹心法原理测定各组标本乙肝表面抗体，按照一定比例稀释样本，直至最后一个稀释度抗HBs含量10IU/L；用LIAISON化学发光免疫分析系统，测定“2和/或3抗体”阳性青年组和老年组标本的巨细胞病毒IgG抗体（CMV-IgG），按照一定比例稀释样本，直至最后一个稀释度抗CMV-IgG14AU/ml。比较分析四组间抗体活性、效价的变化。结果：“2和/或3抗体”阳性青年组和中年组表面抗体水平相比，青年组表面抗体活性水平高于老年组，有统计学显著性差异（P＜0.05）。“2和/或3抗体”阳性青年组和中年组巨细胞病毒IgG抗体活性相比，中年组巨细胞病毒IgG抗体活性高于青年组，有统计学显著性差异（P＜0.05）。青年组单抗体阳性组和“2和/或3抗体”阳组表面抗体活性相比，“2和/或3抗体”阳性组表面抗体活性水平高于单抗体阳组，有统计学显著性差异（P＜0.01）；中年组单抗体阳性组和“2和/或3抗体”阳组表面抗体活性相比，“2和/或3抗体”阳性组表面抗体活性水平高于单抗体阳组，有统计学显著性差异（P＜0.01）结论:乙肝表面抗体随着年龄增长，呈一定的下降趋势。其可能原因是与乙型肝炎病毒感染进程中抗体反应模式有所不同有关。
[Abstract]:Objective: through investigation and analysis, we found that hepatitis B surface antibody with the increase of age has a certain trend of decline, whether this trend is caused by age or other factors, is the main purpose of this study. Methods: the subjects were non-hepatitis patients from the first affiliated Hospital of Dalian Medical University. Hepatitis B surface antibody positive (10 HBs / L, HBsAg 0.05 IUU / L negative, HBeAg 1 IUP / L), aspartate aminotransferase normal (15AST 40U / L, female 15AST 35U / L P / L), alanine aminotransferase (9ALT50UL / L, female 7ALT40UL / L, male 55, male 55). 55 cases of female. The experimental group: according to age and e antibody, core antibody negative or positive was divided into four groups: single antibody positive youth group: age 24-26 years old, negative core antibody, male 10 cases, single antibody positive young group, age 24-26 years old, core antibody negative, male 10 cases, single antibody positive youth group: age 24-26 years old. Female 10. Single antibody positive middle-aged group: age 45-50 years of age negative, core antibody negative. Male 10, female 10. "2 and / or 3" positive youth group: age 24 to 26 years old, or core antibody any positive, Or both are positive. 15 males and 15 females. "2 and / or 3" positive middle-aged group: age 45-50 years of age, either one of them is positive for the antibody or core antibody, or both. 20 cases of female. Using cobas e601 automatic electrochemiluminescence immunoanalyzer, double antibody sandwich method was used to determine the surface antibody of hepatitis B in each group, and the samples were diluted according to a certain proportion. Using the LIAISON chemiluminescence immunoassay system, the cytomegalovirus IgG antibody against cytomegalovirus (CMV-IgG) was detected in the samples of the "2 and / or 3" positive youth group and the elderly group, and the samples were diluted according to a certain proportion. Up to the last dilution, the changes of antibody activity and titer among the four groups were compared and analyzed. Results: the level of surface antibody activity in the young group was higher than that in the elderly group, compared with that in the middle age group and the 2 and / or 3 antibody positive group, the level of surface antibody activity in the young group was higher than that in the old group. There was significant difference (P < 0.05). The activity of cytomegalovirus IgG antibody in middle age group was higher than that in young group, and the activity of IgG antibody in middle age group was higher than that in young group. There was significant difference (P < 0.05). Compared with "2 and / or 3" positive group, the surface antibody activity of "2 and / or 3" positive group was higher than that of single antibody positive group. There was a significant difference (P < 0.01), the surface antibody activity of "2 and / or 3 antibody" positive group was higher than that of single antibody positive group (P < 0.01), and the surface antibody activity of "2 and / or 3 antibody" positive group was higher than that of single antibody positive group (P < 0.01). Conclusion: hepatitis B surface antibody tends to decrease with age, which may be related to the different reaction pattern of antibody in the process of hepatitis B virus infection.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.62

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