荧光定量PCR检测血清miR-122在肝纤维化相关疾病中表达水平的研究

发布时间：2018-03-21 19:47

本文选题：miR-122　切入点：肝纤维化　出处：《长江大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:分析研究正常人和肝纤维化患者、肝硬化患者、肝癌患者血清miR-122表达水平的差异,探讨血清miR-122表达水平与丙氨酸氨基转移酶和天门冬氨酸氨基转移酶的关系,研究miR-122作为肝纤维诊断的血清学标志物的可能性,为临床评估肝脏疾病预后、肝纤维化及早期肝硬化诊断提供指导依据。方法:纳入对照组正常人15例,实验组45例,其中肝纤维化患者、肝硬化患者、肝癌患者各15例。对照组和实验组中乙肝表面抗原阳性患者32例。采取荧光定量PCR检测对照组和实验组患者血清miR122的表达水平,实验组患者检测丙氨酸氨基转移酶和天门冬氨酸氨基转移酶含量。比较不同分组情况下血清miR-122表达水平的差异,分析miR-122与丙氨酸氨基转移酶、天门冬氨酸氨基转移酶相关性。结果:单因素方差分析示正常人、肝纤维化患者、肝硬化患者、肝癌患者四组血清miR-122表达水平不全相同,差异具有统计学意义(F=34.843,P0.05);肝纤维化组、肝硬化组及肝癌组血清miR122含量较对照组均高,其中血清miR-122在肝纤维化组患者中表达水平最高,在肝硬化组和肝癌组之间表达水平无差异;实验组和乙肝表面抗原阳性组患者血清miR-122表达水平亦高于对照组血清。血清miR-122与丙氨酸氨基转移酶、天门冬氨酸氨基转移酶相关系数分别为0.568、0.514。血清mi R-122诊断肝纤维化ROC曲线下面积为0.843±0.064。结论:1.血清miR-122在肝纤维化、肝硬化、肝癌及乙肝表面抗原阳性患者血清中水平均较正常人高。2.荧光定量PCR检测血清miR-122可反映肝脏损伤程度。3.荧光定量PCR检测血清miR-122可作为肝纤维化诊断的潜在血清学标志物。
[Abstract]:Objective: to study the difference of serum miR-122 expression between normal subjects and patients with liver fibrosis, liver cirrhosis and liver cancer, and to explore the relationship between the expression of serum miR-122 and alanine aminotransferase and aspartate aminotransferase. To study the possibility of miR-122 as a serological marker for the diagnosis of hepatic fiber, and to provide guidance for clinical evaluation of the prognosis of liver diseases, liver fibrosis and early cirrhosis. Methods: 15 normal persons were included in the control group and 45 in the experimental group. There were 15 patients with liver fibrosis, 15 patients with liver cirrhosis, 15 patients with liver cancer and 32 patients with positive hepatitis B surface antigen in control group and experimental group. The expression of serum miR122 in control group and experimental group was detected by fluorescence quantitative PCR. The levels of alanine aminotransferase (alt) and aspartate aminotransferase (AST) were measured in the experimental group. The differences of serum miR-122 expression in different groups were compared, and the levels of miR-122 and alanine aminotransferase were analyzed. Results: univariate analysis of variance showed that the levels of serum miR-122 in normal subjects, patients with liver fibrosis, patients with liver cirrhosis and patients with liver cancer were not all the same, and the difference was statistically significant (P < 0.05). The level of serum miR122 in liver cirrhosis group and liver cancer group was higher than that in control group. The expression level of serum miR-122 in liver fibrosis group was the highest, but there was no difference between liver cirrhosis group and liver cancer group. The levels of serum miR-122 in experimental group and hepatitis B surface antigen positive group were also higher than those in control group. Serum miR-122 and alanine aminotransferase, The correlation coefficient of aspartate aminotransferase was 0.568 ~ 0.514. The area under the ROC curve of serum mi R-122 for diagnosing hepatic fibrosis was 0.843 卤0.064. Conclusion: 1. Serum miR-122 in liver fibrosis and cirrhosis. The levels of serum miR-122 in patients with liver cancer and hepatitis B surface antigen positive were higher than those in normal controls. The detection of serum miR-122 by fluorescence quantitative PCR could reflect the degree of liver injury. The detection of serum miR-122 by fluorescence quantitative PCR could be used as a potential serological marker for the diagnosis of hepatic fibrosis.
【学位授予单位】：长江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R575.2

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