IFN-γ基因多态性与青海地区藏族人群乙肝病毒易感性的相关性研究

发布时间：2018-03-23 02:22

本文选题：IFN-γ　切入点：基因多态性　出处：《青海大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：通过青海地区藏族人群中IFN-γ基因多态性的2个位点与慢性乙肝易感性之间的关系，筛选出主要相关位点，为预测青海地区藏族人群的HBV易感性的风险奠定基础。进而为乙肝的临床诊断及预防提供新的理论和依据。方法：（1）研究对象：拟选自2012年6月-2013年9月期间在青海省传染病医院和青海大学附属医院经临床及实验室检查确诊的青海地区慢性乙肝藏族患者250例（病例组）及健康志愿者134例(对照组)为研究对象。（2）基因多态性分析：采用试剂盒提取外周血基因组DNA，采用飞行质谱技术分析检测基因γ-干扰素的+874A/A、+874A/T、+874T/T、+2109C/C、+2109C/T、+2109T/T的基因型。（3）数据分析：计算出各位点基因型及等位基因频率，，分析组间各基因型的差异性，如若P＜0.05，还需应用logistic多元回归，进一步分析各基因型与慢性乙肝易感性之间的关系,若P＞0.05，则基因位点的多态性与乙肝易感性则无关。结果：（1）+2109位点在病例组以及对照组之间的频率分布：①+2109C/C在病例组和对照组分别为10.5%和14.3%，经统计学分析结果表明,各组间的分布频率未见显著性差异；②+2109C/T在病例组和对照组分别为47.3%和43.7%，经统计学分析结果表明,各组间的分布频率未见显著性差异；③+2109T/T在病例组和对照组分别为42.2%和42.1%，经统计学分析结果表明,各组间的分布频率未见显著性差异；（2）+874位点在病例组以及对照组之间的频率分布:①+874A/A在病例组和对照组分别为3.1%和2.4%，经统计学分析结果表明,各组间的分布频率未见显著性差异；②+874A/T在病例组和对照组分别为30.6%和19.8%，经统计学分析结果表明,各组间的分布频率未见显著性差异；③+874T/T在病例组和对照组分别为66.3%和77.8%，经统计学分析结果表明,各组间的分布频率未见显著性差异；（3）+874等位基因T在病例组和对照组基因频率分别为81.6%和87.7%，经统计学分析携带等位基因T的频率升高表明乙肝的易感性越低。结论：(1)IFN-γ+874基因位点多态性与青海地区藏族人群HBV感染的易感性可能无关；(2)IFN-γ+2109基因位点多态性与青海地区藏族人群HBV感染的易感性可能无关；(3)IFN-γ+874的等位基因T可能是青海藏族乙肝的保护性基因。
[Abstract]:Objective: to study the relationship between the two loci of IFN- 纬 gene polymorphism and the susceptibility to chronic hepatitis B in Tibetan population in Qinghai area, and to screen out the main related sites. In order to predict the risk of HBV susceptibility of Tibetan population in Qinghai area, and provide a new theory and basis for clinical diagnosis and prevention of hepatitis B. methods: selected from June 2012 to September 2013 in Qinghai Province. Genetic Polymorphism of 250 Tibetan patients with chronic Hepatitis B (case Group) and 134 healthy volunteers (Control Group) confirmed by Clinical and Laboratory examination in Hospital of Infectious Diseases and affiliated Hospital of Qinghai University. Sex analysis: genomic DNA was extracted from peripheral blood by kit, and 874 A / A, 874 A / T, 874 T / T, 2109 C / T, 2109 C / T, 2109T/T genotypes of interferon 纬 were analyzed by flight mass spectrometry (FMS). If P < 0.05, logistic multiple regression should be used to further analyze the relationship between genotype and chronic hepatitis B susceptibility. If P > 0.05, the polymorphism of the gene locus was not related to the susceptibility to hepatitis B. results the frequency distribution of 2109 2109C/C in the case group and the control group was 10.5% and 14.33%, respectively. There was no significant difference in the frequency of distribution between the two groups (47.3% and 43.7% in the case group and the control group, respectively). The results of statistical analysis showed that there was no significant difference between the two groups. There was no significant difference in the distribution frequency among the three groups (42.2% and 42.1% in the case group and the control group, respectively). There was no significant difference in the distribution frequency between each group and control group. The frequency distribution of 1: 1 874A/A in case group and control group was 3.1% and 2.4%, respectively. The results of statistical analysis showed that there was no significant difference between the two groups. There was no significant difference in the frequency of distribution between each group (30.6%) and control group (19.8%). The results of statistical analysis showed that the frequency of the two groups was 30.6% and 19.8%, respectively. There was no significant difference in the distribution frequency among the three groups (66.3% and 77.8% in the case group and the control group, respectively). There was no significant difference in the distribution of allele T among the three groups. The frequency of allele T was 81.6% in the case group and 87.7% in the control group. The higher the frequency of allele T was, the lower the susceptibility of hepatitis B was. It is suggested that the polymorphism of IFN- 纬 874 gene locus is not related to the susceptibility to HBV infection in Tibetan population in Qinghai area. The polymorphism of IFN- 纬 2109 gene locus may not be related to the susceptibility to HBV infection of Tibetan population in Qinghai area, and the allele T of IFN- 纬 874 may be as follows:. The protective gene of hepatitis B in Qinghai Tibetan nationality.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.62

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