毛细管电泳-发光二极管诱导荧光检测法分析肝硬化及正常人血清蛋白质的差异

发布时间：2018-03-25 05:20

本文选题：毛细管电泳　切入点：发光二极管诱导荧光检测　出处：《色谱》2015年02期

【摘要】：应用毛细管电泳技术建立了人血清蛋白质毛细管电泳分析方法,对比了正常人与肝硬化患者血清蛋白质含量的差异。使用异硫氰酸荧光素(FITC)进行荧光标记,并考察光源与滤光片的波长、缓冲液的浓度、pH值、分离电压对分离效果的影响。建立了毛细管电泳-发光二极管诱导荧光法检测肝硬化患者血清及正常人血清蛋白质的方法;通过分离检测,获得了两者的蛋白质电泳谱图。经分析,在肝硬化患者的血清蛋白质中比在正常人的血清蛋白质中多检测到2个蛋白质峰,可能是和肝硬化发生相关的特异蛋白质;肝硬化患者与正常人的血清蛋白质电泳谱图的差异有统计学意义。该方法能实现人血清蛋白质的分离,可为临床诊断肝硬化做参考。
[Abstract]:A capillary electrophoresis (CE) method for the analysis of human serum protein was established, and the difference of serum protein levels between normal and cirrhotic patients was compared. Fluorescein isothiocyanate (FITC) was used to label human serum protein. The influence of the wavelength of light source and filter, the concentration of buffer and pH value, the separation voltage on the separation effect was investigated. A method of capillary electrophoresis and light-emitting diode induced fluorescence was established for the detection of serum protein in patients with liver cirrhosis and in normal people. Two more protein peaks were detected in the serum proteins of patients with liver cirrhosis than in the normal controls, and the electrophoretic patterns of the two proteins were obtained by separation and detection. It may be a specific protein related to the development of liver cirrhosis, and the difference of serum protein electrophoretic spectrum between patients with cirrhosis and normal persons is statistically significant. This method can realize the separation of human serum proteins and can be used as a reference for clinical diagnosis of liver cirrhosis.
【作者单位】：天津医科大学生物医学工程系;天津市兰力科化学电子高技术有限公司;
【分类号】：R575.2;O657

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