细胞周期蛋白D2对HBV复制的影响

发布时间：2018-03-28 23:23

本文选题：HBV　切入点：cyclin　出处：《第三军医大学学报》2015年15期

【摘要】：目的研究细胞周期蛋白D2(cyclin D2)对HBV复制的影响并探讨其作用的分子机制。方法运用qRT-PCR分析细胞周期蛋白家族成员在HBV复制细胞系中的表达水平;qRT-PCR、Western blot检测HBV复制对cyclin D2表达的影响;Western blot验证cyclin D2沉默效果,Real-time PCR,Southern blot验证沉默cyclin D2对HBV复制中间体表达的影响及ELISA验证cyclin D2对HBs Ag、HBe Ag分泌的影响;并检测cyclin D2过表达对HBV复制的影响。结果在细胞周期蛋白中,HBV复制对cyclin D2表达的影响最为显著,HBV感染明显促进cyclin D2 mRNA和蛋白水平的升高,下调cyclin D2显著抑制HBV的复制中间体的表达,HBs Ag和HBe Ag的分泌,过表达cyclin D2明显促进HBV的复制。结论 HBV复制可以促进细胞内cyclin D2的表达,cyclin D2有利于HBV的复制。这一发现为降低HBV的感染率,探索治疗乙型肝炎新的靶点提供了新的策略。
[Abstract]:Objective to study the effect of cyclin D2(cyclin D2 (D2(cyclin D2) on HBV replication and its molecular mechanism. Methods qRT-PCR was used to analyze the expression of cyclin family members in HBV replication cell line and qRT-PCRG Western blot was used to detect the effect of HBV replication on cyclin. The effect of cyclin D2 silencing was verified by Western blot. The effect of cyclin D2 silencing on the expression of HBV replication intermediates and the effect of cyclin D2 on HBs AgHBeAg secretion were verified by Southern blot. The effect of cyclin D2 overexpression on HBV replication was also detected. Results in cyclin, the effect of HBV replication on cyclin D2 expression was most significant. HBV infection significantly promoted the increase of cyclin D2 mRNA and protein levels. Down-regulation of cyclin D2 significantly inhibited the expression of HBV replication intermediates and the secretion of HBs Ag and HBe Ag. Conclusion the overexpression of cyclin D2 can promote the replication of HBV. Conclusion HBV replication can promote the replication of cyclin D2 in cells. This discovery provides a new strategy for reducing the infection rate of HBV and exploring new targets for the treatment of hepatitis B.
【作者单位】：重庆医科大学感染性疾病分子生物学教育部重点实验室;
【基金】：国家自然科学基金面上项目(81472271,81201282)~~
【分类号】：R512.62

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