α-硫辛酸对四氯化碳诱导小鼠肝纤维化的保护作用

发布时间：2018-04-02 04:04

本文选题：α-硫辛酸　切入点：肝纤维化　出处：《中国老年学杂志》2017年06期

【摘要】：目的研究α-硫辛酸(LA)对四氯化碳(CCl4)诱导小鼠肝纤维化的保护作用及机制。方法 75只ICR雄性小鼠随机分为正常组、模型组、阳性对照组(水飞蓟素)和LA低、高剂量组。自造模之日起,除正常组外,其余各组以2.5 ml/kg腹腔注射20%CCl4,每周2次,连续6 w。自造模之日起,LA低、高剂量组分别给予(100,300 mg·kg~(-1)·d~(-1))灌胃,正常组和模型组小鼠给予1.0 g·kg~(-1)·d~(-1)的生理盐水灌胃,阳性对照组给予水飞蓟素(200 mg·kg~(-1)·d~(-1))灌胃,连续给药42 d。末次给药2 h后乙醚麻醉,从眼内眦静脉取血,摘取肝脏,比较各组小鼠肝功能指标和肝脏组织病理学特征的差异。结果 LA低、高剂量组谷草转氨酶(AST)、谷丙转氨酶(ALT)活性明显低于模型组(P0.01);肝组织中超氧化物歧化酶(SOD)活性较模型组明显升高(P0.01);丙二醛(MDA)含量显著下降(P0.01);通过Masson、HE染色光镜下观察组织病理学变化,观察到LA低、高剂量组可使炎细胞浸润程度、肝细胞水肿程度及肝脏纤维化程度较模型组有所减轻。结论 LA可明显减轻CCl4诱导的小鼠肝纤维化及肝脏炎症反应,其机制可能与抗氧化损伤有关。
[Abstract]:Objective to study the protective effect and mechanism of 伪 -lipoic acid (伪 -lipoic acid) on CCl4) induced liver fibrosis in mice. Methods 75 ICR male mice were randomly divided into normal group, model group, positive control group (silymarin) and low LA. From the day of making the model, the other groups were given 2.5 ml/kg intraperitoneal injection of 20l4 twice a week for 6 weeks. Mice in normal group and model group were given normal saline (1.0 g 路kg ~ (-1)) 路d ~ (-1) by gavage, and positive control group were given silymarin (200 mg 路kg ~ (-1)) 路d ~ (-1) by gavage. After the last administration for 42 days, the mice were anesthetized with ether, and blood was taken from canthus intraocular vein and liver was obtained. The difference of liver function index and liver histopathological characteristics in each group was compared. The activity of glutamic oxaloacetic transaminase (AST) and alanine aminotransferase (alt) in high dose group was significantly lower than that in model group (P 0.01), the activity of superoxide dismutase (SOD) in liver tissue was significantly higher than that in model group (P 0.01), and the content of malondialdehyde (MDA) was significantly decreased (P 0.01). Histopathological changes, It was observed that the degree of inflammatory cell infiltration, the degree of hepatic edema and the degree of hepatic fibrosis in the high dose group were lower than those in the model group. Conclusion LA can significantly reduce the hepatic fibrosis and inflammation induced by CCl4 in mice. The mechanism may be related to antioxidant damage.
【作者单位】：江南大学无锡医学院;阜阳市人民医院特需病房;
【基金】：中央高校基本科研业务费专项资金资助(No.JUSRP51412B)
【分类号】：R575.2

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