2型糖尿病患者PEDF基因启动子区多态性与非酒精性脂肪肝病的相关性

发布时间：2018-04-03 09:24

本文选题：型糖尿病　切入点：色素上皮衍生因子　出处：《南方医科大学学报》2015年07期

【摘要】：目的探讨福建地区汉族2型糖尿病患者血清色素上皮衍生因子水平及色素上皮衍生因子基因启动子区-358位点单核苷酸多态性与非酒精性脂肪肝病的关系。方法选择2型糖尿病患者共452例,按有无合并非酒精性脂肪肝病分为性别、年龄比例相近的DM1组(合并非酒精性脂肪肝病)282例,DM2组(不合并非酒精性脂肪肝病)170例,采用聚合酶链反应——限制性片段长度多态性(PCR-RFLP)技术检测色素上皮衍生因子基因启动子区-358位点多态性,同时检测血清色素上皮衍生因子水平及空腹血糖、空腹胰岛素、糖化血红蛋白等指标。结果 DM1组的血清色素上皮衍生因子水平高于DM2组,差异有统计学意义(P0.05);以非酒精性脂肪肝病为因变量的二分类非条件Logistic回归分析中,结果显示血清色素上皮衍生因子作为独立影响因素影响2型糖尿病患者非酒精性脂肪肝病的发生;色素上皮衍生因子基因启动子区SNP-358G→A的基因型(GG型、GA型和AA型)和等位基因(G/A)在DM1、DM2两组间的分布存在差异,有统计学意义(P0.05);2型糖尿病患者-358位点GA基因型出现非酒精性脂肪肝病的风险是GG基因型的2.032倍,GA+AA基因型出现非酒精性脂肪肝病的风险是GG基因型的2.068倍,差异有统计学意义(P0.05)。结论血清色素上皮衍生因子水平是2型糖尿病患者发生非酒精性脂肪肝病的独立影响因素,血清色素上皮衍生因子水平的升高为胰岛素抵抗的反馈表现,可改善胰岛素抵抗,保护胰岛素敏感性。2型糖尿病患者的色素上皮衍生因子基因启动子区-358G→A多态性与非酒精性脂肪肝病有关,携带A等位基因可能增加非酒精性脂肪肝病的易感性。
[Abstract]:Objective to investigate the relationship between serum pigment epithelium-derived factor level, -358 locus polymorphism in promoter region of pigment epithelium-derived factor gene and non-alcoholic fatty liver disease in Fujian Han nationality patients with type 2 diabetes mellitus.Methods A total of 452 patients with type 2 diabetes mellitus were divided into two groups according to whether they had non-alcoholic fatty liver disease or not. The patients were divided into two groups: DM1 group (n = 282) with non-alcoholic fatty liver disease (n = #number1#).Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the polymorphism of -358 locus in the promoter region of pigment epithelium-derived factor gene. Serum pigment epithelium-derived factor level, fasting blood glucose and fasting insulin were also detected.Glycosylated hemoglobin and other indicators.Results the level of serum pigment epithelium derived factor in DM1 group was higher than that in DM2 group, and the difference was statistically significant (P 0.05).The results showed that serum pigment epithelium-derived factor was an independent factor affecting the occurrence of non-alcoholic fatty liver disease in patients with type 2 diabetes mellitus.There were differences in the distribution of GG type GA and AA genotype in the promoter region of pigment epithelium-derived factor gene (SNP-358G) and the distribution of allele G / A in DM1 / DM2 group.The risk of non-alcoholic fatty liver disease was 2.032 times of GG genotype and 2.068 times higher than that of GG genotype in patients with type 2 diabetes mellitus (P 0.05). The difference was statistically significant (P 0.05).Conclusion the level of serum pigment epithelium-derived factor is an independent factor influencing the occurrence of non-alcoholic fatty liver disease in patients with type 2 diabetes mellitus. The increase of serum pigment epithelium-derived factor level is a feedback sign of insulin resistance, which can improve insulin resistance.The polymorphisms in the promoter region of pigment epithelium-derived factor gene in insulin-sensitive type 2 diabetic patients were associated with non-alcoholic fatty liver disease, and the allele A might increase the susceptibility of non-alcoholic fatty liver disease.
【作者单位】：泉州医学高等专科学校内科学教研室;福建医科大学附属第二医院内分泌科;
【基金】：福建省教育厅科技项目(JA13396)
【分类号】：R587.1;R575.5

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