溃疡性结肠炎大鼠血清白细胞介素-1β、肿瘤坏死因子-α、白细胞介素-4动态表达规律

发布时间：2018-04-04 06:20

  本文选题：溃疡性结肠炎(UC)　切入点：白细胞介素-β　出处：《中国老年学杂志》2015年02期

【摘要】：目的探讨溃疡性结肠炎(UC)大鼠血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、IL-4的动态表达规律。方法将60只大鼠随机分为空白组10只、乙醇组10只,模型组40只(3、7、14、21 d各10只),采用TNBS/乙醇灌肠法制备溃疡性结肠炎大鼠模型,于制模后3、7、14、21 d 4个时间点采用ELISA法检测大鼠血清IL-1β、TNF-α、IL-4水平,光镜观察结肠组织形态并评分。结果制模后3 d大鼠结肠出现炎症和溃疡,且随着时间推移而加重,7~14 d溃疡和炎症最为严重,21 d溃疡和炎症已经有所修复。模型组各时间点大鼠血清促炎因子IL-1β、TNF-α均高于空白组和乙醇组,抗炎因子IL-4水平均低于空白组和乙醇组,以7 d和14 d升高或降低更为显著(P0.05、P0.01);7、14 d组与3d组比较,IL-1β、TNF-α水平进一步升高(P0.01),而IL-4水平进一步降低(P0.01);21 d组与7、14 d组比较,IL-1β、TNF-α水平下降,而IL-4水平则升高(P0.05)。结论促炎因子IL-1β、TNF-α和抗炎因子IL-4在UC发病中起重要作用,UC的发病及严重程度与促炎因子IL-1β、TNF-α、IL-4的动态表达及失衡密切相关。
[Abstract]:Objective to investigate the dynamic expression of IL-4 in serum of rats with ulcerative colitis (UC) and tumor necrosis factor- 伪 (TNF- 伪).Methods 60 rats were randomly divided into blank group (n = 10), ethanol group (n = 10) and model group (n = 40). The ulcerative colitis model was established by TNBS/ ethanol enema.The serum levels of TNF- 伪 IL-4 in serum of rats were measured by ELISA method at 4 time points of 21 days after 3 days, 7 days and 14 days, and the colonic tissue morphology was observed by light microscope and evaluated.Results the colonic inflammation and ulceration appeared in rats 3 days after the model making, and the ulcer and inflammation were aggravated at 714 days and the most serious ulcer and inflammation were repaired in 21 days.The serum levels of IL-1 尾 -TNF- 伪 in model group were higher than those in control group and ethanol group, and the level of anti-inflammatory factor IL-4 in model group was lower than that in blank group and ethanol group.浠，

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