P38MAPK调控重症急性胰腺炎大鼠海马神经元COX-2和PGE2表达的研究

发布时间：2018-04-04 14:23

本文选题：重症急性胰腺炎　切入点：脑损害　出处：《神经解剖学杂志》2015年05期

【摘要】：目的:研究P38丝裂原活化蛋白激酶(P38MAPK)对重症急性胰腺炎(SAP)大鼠海马神经元环氧合酶-2(COX-2)和前列腺素E2(PGE2)表达的调控作用。方法:雄性健康SD大鼠36只,体重220~260 g,随机分为3组。SAP模型组:采用向胰胆管内注入5%牛磺胆酸钠(2 mg/kg)的方法制备SAP动物模型;抑制剂组:SAP建模成功5 min后,大鼠尾静脉注射P38MAPK抑制剂SB203580(10 mg/kg);对照组:行剖腹术翻动胰腺和十二指肠后缝合腹腔。各组大鼠分别于术后24 h,用Nissl染色和免疫组化法观察脑组织病理改变的情况,用Western Blot检测脑组织中p-P38蛋白、COX-2和PGE2的表达情况。结果:模型组大鼠海马CA1区局部锥体细胞缺失,p-P38、COX-2和PGE2阳性细胞数明显增加(P0.01),且相应蛋白表达显著升高(P0.01);SB203580处理组以上变化明显减轻或不明显(P0.01)。结论:P38MAPK对实验SAP大鼠海马COX-2和PGE2表达具有显著调控作用,而P38MAPK抑制剂SB203580则对SAP大鼠海马神经元具有一定保护作用。
[Abstract]:Aim: to investigate the effects of P38 mitogen-activated protein kinase (P38 MAPK) on the expression of cyclooxygenase-2 (COX-2) and prostaglandin E _ 2 (PGE2) in hippocampal neurons of rats with severe acute pancreatitis (SAP).Methods: 36 healthy male SD rats, weighing 220 ~ 260 g, were randomly divided into 3 groups. SAP model group was established by injecting 5% sodium taurocholate 2 mg / kg into the pancreaticobiliary duct, and the SAP model was established by injection of 5% sodium taurocholate into the pancreatic duct, and 5 min after injection of 5% sodium taurocholate into the pancreatic duct.P38MAPK inhibitor SB203580(10 mg / kg was injected into caudal vein of rats, while in control group, abdominal cavity was sutured after operation of pancreas and duodenum.The pathological changes of brain tissue were observed by Nissl staining and immunohistochemistry at 24 hours after operation. The expression of p-P38 protein COX-2 and PGE2 in brain tissue was detected by Western Blot.Results: the number of COX-2 and PGE2 positive cells in the local pyramidal cells of hippocampal CA1 in the model group was significantly increased, and the corresponding protein expression was significantly increased in the P0.01SSB203580 treated group.Conclusion the expression of COX-2 and PGE2 in hippocampus of experimental SAP rats was significantly regulated by P38 MAPK, while P38MAPK inhibitor SB203580 could protect hippocampal neurons in SAP rats.
【作者单位】：第四军医大学唐都医院消化科;解放军第323医院肝胆外科;第四军医大学西京医院消化科;
【基金】：国家自然科学基金(30872965,30971337)
【分类号】：R576

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