自制KYL器官保存液对恒河猴肝脏的保存作用

发布时间：2018-04-05 02:23

本文选题：组织工程　切入点：肝移植　出处：《中国组织工程研究》2015年24期

【摘要】：背景:目前肝脏移植是治疗终末期肝病的最佳方法,UW液是公认的肝脏保存液,但是其价格昂贵,并且细胞外液高K+状态,与人的生理不符,常会导致患者瞬时高钾血症,引起心跳停止,因此研发新的器官保存液中已经刻不容缓。文文目的:观察自制KYL液对恒河猴肝脏缺血再灌注后的保存效果。(方法:实验选受体猴和供体猴各8只,每组分别包括KYL液组和UW液组各4例供体肝脏灌注并冷保存4 h,稿行同种异体原位肝移植。分别于移植后30 min、6 h,记录胆汁分泌量,采血检测谷草转氨酶、谷丙转氨酶、h一氧化氮、内皮素1、肿瘤坏死因子α浓度,切取肝组织行光学显微镜检测。结果与结论:两组移植后均有胆汁分泌,随时间延长胆汁分泌量增加(P0.05)。移植后30 min、6 h,KYL液组血清谷草转氨酶和谷丙转氨酶浓度均低于UW液组(P0.05),两组血清一氧化氮、内皮素1、肿瘤坏死因子α浓度差异无显著性意义(P0.05)。光学显微镜下肝组织形态学观察显示,UW液组较KYL液组肝细胞水肿明显。结果证实,KYL液在预防缺血再灌注损伤效果与UW液总体相当,部分效果略优于UW液。
[Abstract]:Background: at present, liver transplantation is the best method for the treatment of end-stage liver disease. However, the UW solution is recognized as a liver preservative, but its price is high, and the extracellular fluid is high in K state, which is not in accordance with human physiology, and often leads to transient hyperkalemia in patients.The heartbeat stops, so it is urgent to develop new organ preservatives.Objective: to observe the preservation effect of self-made KYL solution on rhesus monkey liver ischemia reperfusion.Methods: eight recipient monkeys and eight donor monkeys were selected in the experiment. Each group consisted of KYL solution group and UW liquid group respectively. The donor liver was perfused and cold preserved for 4 hours, and allograft orthotopic liver transplantation was performed in each group.Bile secretion was recorded at 30 min after transplantation. Blood samples were collected for detection of glutamic oxalacetic transaminase (alt), nitric oxide (no), endothelin 1 (et 1), tumor necrosis factor 伪 (TNF- 伪).Results and conclusion: bile secretion was found in both groups after transplantation, and the amount of bile secretion increased with time.The concentrations of serum glutamic oxaloacetic transaminase and alanine aminotransferase in KYL solution group were lower than those in UW solution group at 30 min after transplantation. There was no significant difference in serum nitric oxide, endothelin 1 and tumor necrosis factor 伪 concentration between the two groups.The morphological observation of liver tissue under optical microscope showed that the edema of liver cells in UW liquid group was more obvious than that in KYL solution group.The results showed that the effect of Kyl solution on the prevention of ischemia reperfusion injury was similar to that of UW solution, and the partial effect was slightly better than that of UW solution.
【作者单位】：昆明市第一人民医院;
【分类号】：R575

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