肝脂肪酸结合蛋白在乙型肝炎相关性肝细胞癌中的作用及机制

发布时间：2018-04-08 22:40

本文选题：乙型肝炎病毒　切入点：乙型肝炎X蛋白　出处：《中南大学》2014年硕士论文

【摘要】：背景和目的：慢性乙型肝炎病毒(HBV)感染被认为是肝细胞癌发生发展的主要原因,其中乙肝病毒X蛋白(HBx)在其中扮演着重要的角色。前期的研究表明肝脂肪变性是肝细胞癌的一个重要风险因素,HBx可诱导肝脂肪变性。然而,肝脏脂肪酸结合蛋白(L-FABP)是肝细胞中脂质信号传导及脂代谢中重要的脂质信号转导蛋白。而HBx与L-FABP的相关性及L-FABP在肝细胞癌发生发展的作用及其机制尚未明了。在本研究中,我们研究了HBx和L-FABP的相关性及FABP在乙肝病毒诱导的肝癌中的作用机理。方法：我们将编码HBx质粒转染人肝癌细胞株HepG2细胞,用蛋白免疫印迹法检测L-FABP表达。随后,转染L-FABP RNAi质粒阻止L-FABP的表达,用油红O染色比较转染L-FABP沉默质粒及未转染细胞内脂质变性的差异,MTT及DAPI染色检测细胞增殖能力,流式细胞术检测细胞周期和细胞凋亡。结果：免疫蛋白印迹结果显示转染HBx的细胞L-FABP的表达高于未转染细胞,油红O染色结果显示转染L-FABP RNAi质粒细胞内脂滴生成明显少于未转染细胞,MTT及DAPI染色显示转染L-FABP RNAi质粒的细胞增殖能力显著低于对照组。结论：我们的研究结果显示,HBx可以上调L-FABP的表达,L-FABP在促进脂肪的生成的同时,可通过促进细胞增殖和抑制细胞凋亡的途径影响肝细胞癌的发生发展。图12幅,表3个,参考文献69篇
[Abstract]:Background & objective: chronic hepatitis B virus (HBV) infection is considered to be the main cause of the development of hepatocellular carcinoma, in which hepatitis B virus X protein (HBX) plays an important role.Previous studies have shown that hepatic steatosis is an important risk factor for hepatocellular carcinoma.However, liver fatty acid binding protein (L-FABP) is an important lipid signal transduction protein in hepatocyte lipid signal transduction and lipid metabolism.However, the relationship between HBx and L-FABP, the role of L-FABP in the development of hepatocellular carcinoma and its mechanism are not clear.In this study, we studied the correlation between HBx and L-FABP and the mechanism of FABP in Hepatitis B induced liver cancer.Methods: human hepatoma cell line HepG2 cells were transfected with encoding HBx plasmid and the expression of L-FABP was detected by Western blot.Then, the expression of L-FABP was blocked by transfection of L-FABP RNAi plasmid. The difference between transfected L-FABP silencing plasmid and untransfected cell lipids denaturation was compared by oil red O staining. Cell proliferation ability was detected by DAPI staining, cell cycle and apoptosis were detected by flow cytometry.Results: the results of Western blot showed that the expression of L-FABP in HBx transfected cells was higher than that in untransfected cells.The results of oil red O staining showed that lipid droplet formation in transfected L-FABP RNAi plasmid was significantly lower than that in untransfected cells. DAPI staining showed that the proliferation ability of transfected L-FABP RNAi plasmid was significantly lower than that of control group.Conclusion: our results suggest that HBX can up-regulate the expression of L-FABP and L-FABP may affect the development of hepatocellular carcinoma by promoting cell proliferation and inhibiting apoptosis.12 figures, 3 tables, 69 references
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.62;R735.7

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