急性胰腺炎中E-钙粘蛋白的诊断价值

发布时间：2018-04-09 21:04

本文选题：胰腺炎　切入点：诊断　出处：《重庆医学》2015年02期

【摘要】：目的探讨E-钙粘蛋白(E-cad)在急性胰腺炎中的表达及对其严重性的诊断价值。方法 24只雄性SD大鼠随机均分为对照组、轻症急性胰腺炎组(MAP组)和重症急性胰腺炎组(SAP组)。通过腹腔注射L-精氨酸造模,建模24h后测定血清中淀粉酶活性及E-cad表达变化,胰腺组织进行病理组织学观察,蛋白免疫印迹(Western blot)检测胰腺组织E-cad的蛋白表达。结果 MAP组和SAP组胰腺病理评分、血清淀粉酶值明显高于对照组(P0.05),SAP组与MAP组比较差异也有统计学意义(P0.05);血清E-cad酶联免疫吸附试验(ELISA)检测结果显示:MAP组(1 025.50±131.33)pg/mL和SAP组(1 561.75±144.82)pg/mL均明显高于对照组(626.50±72.12)pg/mL,且两组之间比较差异也有统计学意义(P0.05);Western blot检测胰腺组织中SAP组E-cad表达强于对照组及MAP组。结论 E-cad在急性胰腺炎早期表达明显增高,对其严重性具有一定的诊断价值。
[Abstract]:Objective to explore the value of the expression of E- cadherin (E-cad) in acute pancreatitis and the severity of their diagnosis. Methods 24 male SD rats were randomly divided into control group, mild acute pancreatitis group (MAP group) and severe acute pancreatitis group (SAP group). By intraperitoneal injection of L- arginine modeling. The expression changes of amylase activity and serum E-cad determination of 24h after modeling, histopathological observation of pancreatic tissue by Western blotting (Western blot) to detect the expression of pancreatic tissue E-cad protein. The results of MAP group and SAP group, pancreatic pathological score, serum amylase values were significantly higher than the control group (P0.05), there were significant differences between group SAP and group MAP group (P0.05); serum E-cad enzyme linked immunosorbent assay (ELISA) test results show that: the MAP group (1025.50 + 131.33) pg/mL and SAP group (1561.75 + 144.82) pg/mL were significantly higher than the control group (626.50 + 72.12) pg/mL, and the two group There was statistically significant difference between the two groups (P0.05). The expression of E-cad in the SAP group was stronger than that in the control group and MAP group by Western blot test. Conclusion E-cad expression in the early stage of acute pancreatitis is significantly higher, and it has certain diagnostic value for its severity.

【作者单位】：宜兴市人民医院消化科;上海交通大学医学院;
【分类号】：R576

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