乙型肝炎病毒影响肝癌HepG2细胞代谢的气相色谱-质谱研究

发布时间：2018-04-10 03:07

本文选题：乙型肝炎病毒　切入点：HepG细胞　出处：《上海交通大学学报(医学版)》2017年10期

【摘要】：目的·研究乙型肝炎病毒(HBV)感染对肝癌HepG2细胞代谢的影响。方法·采用气相色谱质谱联用技术(GC-MS)检测肝癌HepG2细胞和HepG2.2.15细胞(HepG2细胞的衍生细胞)的代谢物组成,分析其差异。结果·GC-MS分析发现HepG2及HepG2.2.15细胞的亲水性提取物中主要含有34种代谢物,包括甘氨酸、丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、丝氨酸、苏氨酸、甲硫氨酸、半胱氨酸、胱氨酸、天冬氨酸、谷氨酸、焦谷氨酸、苯丙氨酸、酪氨酸、色氨酸、次黄嘌呤、尿嘧啶、肌醇、乳酸、丁二酸、亚油酸、亚麻酸、软脂酸、硬脂酸、尿素和胆固醇等,覆盖糖酵解、脂肪酸代谢、氨基酸代谢、嘌呤与嘧啶代谢等多条途径。HepG2.2.15细胞中乳酸、亚麻酸、丙氨酸、半胱氨酸的含量显著低于HepG2细胞,亮氨酸、异亮氨酸、缬氨酸、苯丙氨酸、甲硫氨酸、色氨酸、脯氨酸、酪氨酸、肌醇、尿嘧啶等代谢物含量显著高于HepG2细胞。结论·HBV感染会导致HepG2细胞氨基酸代谢和脂类代谢改变,基于GC-MS的代谢组学技术能为HBV感染的代谢应答机制提供补充信息。
[Abstract]:Objective to study the effect of hepatitis B virus (HBV) infection on the metabolism of hepatocellular carcinoma (HepG2) cells.Methods Gas chromatography-mass spectrometry (GC-MS) was used to detect the metabolites of hepatoma HepG2 cells and HepG2.2.15 cells derived from HepG2 cells.Results GC-MS analysis showed that the hydrophilic extracts of HepG2 and HepG2.2.15 cells mainly contained 34 metabolites, including glycine, alanine, valine, leucine, isoleucine, proline, serine, threonine, methionine, cysteine.Cystine, aspartic acid, glutamate, pyroglutamic acid, phenylalanine, tyrosine, tryptophan, Hypoxanthine, uracil, inositol, lactic acid, succinic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, urea and cholesterol, etc.,The contents of lactic acid, linolenic acid, alanine and cysteine in HepG2.2.15 cells were significantly lower than those in HepG2 cells, leucine, isoleucine and valine.The contents of phenylalanine, methionine, tryptophan, proline, tyrosine, inositol and uracil were significantly higher than those in HepG2 cells.Conclusion HBV infection may lead to the changes of amino acid and lipid metabolism in HepG2 cells. The metabolomics based on GC-MS can provide supplementary information for the metabolic response mechanism of HBV infection.
【作者单位】：复旦大学生命科学学院;
【基金】：国家自然科学基金(91439102,81590953)~~
【分类号】：O657.63;R512.62

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