亚慢性暴露壬基酚诱发大鼠非酒精性脂肪肝的研究

发布时间：2018-04-10 16:33

本文选题：壬基酚 + 非酒精性脂肪肝　；参考：《重庆医学》2016年28期

【摘要】：目的探讨连续90d灌胃壬基酚(NP)能否诱发非酒精性脂肪肝(NAFLD)。方法 48只雄性SD大鼠分成4组,即对照组,低、中、高剂量组,NP剂量分别为0、20、60、180mg·kg-1·d-1,每组平均分为常规饮食、高脂饮食两个亚组。空腹灌胃NP 90d,期间每周称量1次体质量,第60天行肝脏彩超观察。第90天全自动生化分析仪检测肝功、血脂,高效液相色谱仪检测肝脏中NP的浓度。结果各剂量常规饮食组与对照组比较,低、中、高剂量组三酰甘油(TG)降低(P0.05),天门冬酸氨基转移酶(AST)升高(P0.05),低、高剂量组丙氨酸氨基转移酶(ALT)降低(P0.05),低密度脂蛋白(LDL)减低(P0.05),对照组及低、中剂量组未出现脂肪肝,高剂量组1/2的动物出现脂肪肝。各剂量高脂饮食组与对照比较,低、中、高剂量组、TG升高(P0.05),ALT降低(P0.05),AST升高(P0.05),HDL升高(P0.05),高剂量组总胆固醇(TC)升高(P0.05),高脂饮食组被选部分动物中,低、中剂量组1/2的动物出现了脂肪肝,高剂量组则全部出现脂肪肝病变。与对照组比较,低、中、高剂量组大鼠肝脏NP浓度升高(P0.05),肝脏NP浓度与染毒剂量的存在秩相关rs常规饮食=0.942,rs高脂饮食=0.481(P0.05)。结论亚慢性染暴露NP是导致NAFLD的危险因素之一,增加了NAFLD的发病风险。
[Abstract]:Objective to investigate whether nonylphenol (NPN) can induce NAF LDD in non alcoholic fatty liver for 90 days.Methods 48 male Sprague-Dawley rats were divided into 4 groups: control group, low dose group, middle dose group and high dose group (n = 4). The dosage of NP was 0 ~ 200.60180mg kg-1 路d ~ (-1), respectively. Each group was divided into two subgroups: conventional diet and high-fat diet.The body mass was measured once a week and observed by color Doppler ultrasound on the 60th day.Liver function, blood lipid and NP concentration in liver were detected by automatic biochemical analyzer on day 90.Results compared with the control group, the levels of triacylglycerol (TG) and aspartate aminotransferase (AST) in each dose group were lower than those in the control group, and the levels of triglyceride TGG and aspartate aminotransferase (AST) were lower and lower than those in the control group.In high dose group, alanine aminotransferase (alt) decreased P0.05N, low density lipoprotein (LDL) decreased P0.05n. In control group and low dose group, fatty liver was not found in middle dose group, and fatty liver was found in 1 / 2 of animals in high dose group.Fatty liver lesions were found in the high dose group.Compared with the control group, the liver NP concentration in the low, middle and high dose groups was higher than that in the control group (P 0.05). There was a rank correlation between the liver NP concentration and the exposure dose. There was a rank correlation between the liver NP concentration and the exposure dose.Conclusion Subchronic exposure to NP is one of the risk factors for NAFLD and increases the risk of NAFLD.
【作者单位】：遵义医学院公共卫生学院;遵义医学院附属医院胃肠外科;
【基金】：国家自然科学基金资助项目(201360439,81560527) 贵州省教育厅青年基金资助项目(黔教合KY字[2013]198) 贵州省卫生厅基金资助项目(gzwkj20131127) 遵义医学院招标基金资助项目(2013F-68);遵义医学院研究生社会实践基金资助项目(ysh-2015-001) 贵州省科技厅基金资助项目(黔科合LH字[2014]7543,黔科合J字[2014]2177号,[2014]2185号)
【分类号】：R575.5

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