青蒿琥酯对非酒精性脂肪肝小鼠肝脏TLR4及MyD88表达的影响

发布时间：2018-04-11 22:26

  本文选题：青蒿琥酯 + 非酒精性脂肪性肝病　； 参考：《安徽医科大学学报》2017年10期

【摘要】：目的观察青蒿琥酯对非酒精性脂肪肝病(NAFLD)小鼠肝脏Toll样受体4(TLR4)、髓样分化因子88(My D88)和磷脂酰肌醇-3激酶(PI3K)表达的影响。方法 70只昆明小鼠随机分为正常对照组、模型组、青蒿琥酯高[60 mg/(kg·d)]、中[30 mg/(kg·d)]、低[15 mg/(kg·d)]剂量组。采用高脂饲料构建NAFLD小鼠动物模型,于给药后12周末处死,观察肝脏病理变化,酶法检测血清三酰甘油(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)水平,RT-PCR法检测肝组织TLR4、My D88、PI3K mRNA表达。结果各青蒿琥酯剂量组小鼠血清TG、TC和ALT水平比模型组显著降低,肝脏脂肪变性明显减轻,肝组织TLR4、My D88及PI3K表达量也显著降低(P0.01,P0.05)。与正常对照组相比,模型组肝组织TLR4、My D88及PI3K表达量显著升高(P0.01),且三者有相同的变化趋势。结论 TLR4/My D88及其下游PI3K/AKT通路可能在NAFLD发病中起重要作用。青蒿琥酯可通过降低TLR4、My D88、PI3K mRNA表达,减轻NAFLD小鼠肝脏脂肪变性程度,降低血脂,改善肝功能指标。
[Abstract]:Objective to observe the effects of artesunate on the expression of Toll like receptor 4TLR4 (myeloid differentiation factor 88(My D88) and phosphatidylinositol 3 kinase (PI3K) in the liver of mice with nonalcoholic fatty liver disease (NAF LDD).Methods 70 Kunming mice were randomly divided into normal control group, model group, artesunate high [60 mg/(kg d], middle [30 mg/(kg d], low [15 mg/(kg d] group.The NAFLD mouse model was established with high fat diet. The liver pathological changes were observed at the end of 12 weeks after administration. The levels of serum triglyceride, total cholesterol and alanine aminotransferase (alt) were detected by enzyme method. The expression of TLR4My D8PI3K mRNA in liver tissue was detected by RT-PCR.Results compared with the model group, the levels of serum TC and ALT in each artesunate group were significantly lower than those in the model group, and the liver steatosis was significantly reduced. The expression of TLR4My D88 and PI3K in the liver tissue was also significantly decreased compared with the model group.Compared with the normal control group, the expression of TLR4My D88 and PI3K in the model group was significantly higher than that in the normal control group.Conclusion TLR4/My D 88 and its downstream PI3K/AKT pathway may play an important role in the pathogenesis of NAFLD.Artesunate could reduce the degree of hepatic steatosis, decrease blood lipid and improve liver function index by decreasing the expression of TLR4My D88PI3K mRNA in liver of NAFLD mice.
【作者单位】： 桂林医学院附属医院研究生科;桂林医学院附属医院感染性疾病科;桂林医学院附属医院新生儿科;桂林医学院附属医院老年内科;
【基金】：国家自然科学基金(编号:81260078) 广西自然科学基金(编号:2012GXNSFAA276038、2014GXNSFAA118152) 广西医药卫生自筹经费计划课题(编号:Z2015367)
【分类号】：R575.5

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