Hp感染萎缩性胃炎胃黏膜中T淋巴细胞亚群及PD-1表达的意义

发布时间：2018-04-13 07:29

本文选题：幽门螺杆菌 + T淋巴细胞亚群　；参考：《蚌埠医学院》2017年硕士论文

【摘要】：目的检测人Hp感染的慢性胃炎(浅表性胃炎、萎缩性胃炎)T淋巴细胞亚群及PD-1的表达,探讨人Hp感染的慢性胃炎(浅表性胃炎、萎缩性胃炎)胃黏膜免疫损伤机制,为临床应用提供依据。方法经过解放军第九七医院伦理委员会审批,患者签署知情同意书。收集胃镜下钳取40例Hp阳性胃炎(Hp阳性浅表性胃炎20例、Hp阳性萎缩性胃炎20例),40例Hp阴性胃炎(Hp阴性浅表性胃炎20例、Hp阴性萎缩性胃炎20例)胃黏膜组织标本。采用免疫组织化学方法检测CD4+T细胞、CD8+T细胞、Foxp3、IL-17及PD-1的表达。Hp阴性、Hp阳性根据14C呼气试验和HE染色进行判断,双阳性为阳性组、双阴性为阴性组。通过Image-pro plus图像分析系统测定检测指标累积光密度值(IOD)。结果1.Hp阳性萎缩性胃炎胃黏膜组中CD4+T细胞、CD8+T细胞、Foxp3、IL-17及PD-1的表达高于Hp阴性萎缩性胃炎胃黏膜组,差异有统计学意义(P0.05)。2.Hp阳性萎缩性胃炎组中CD4+T细胞、CD8+T细胞、Foxp3、IL-17及PD-1的表达高于Hp阳性浅表性胃炎组,差异具有统计学意义(P0.05)。3.Hp阳性胃炎(浅表+萎缩)胃黏膜组中CD4+T细胞、CD8+T细胞、Foxp3、IL-17及PD-1的表达量高于Hp阴性胃炎(浅表+萎缩)胃黏膜组,差异具有统计学意义(P0.05)。4.Hp阳性浅表性胃炎胃黏膜组中CD4+T细胞、IL-17及PD-1的表达高于Hp阴性浅表性胃炎胃黏膜组,差异有统计学意义(P0.05)。5.Hp阳性浅表性胃炎胃黏膜组中CD8+T细胞和Foxp3的表达高于Hp阴性浅表性胃炎胃黏膜组,差异无统计学意义(P0.05)。结论1.Hp感染阳性胃炎胃黏膜中T淋巴细胞亚群(CD4+T细胞、CD8+T细胞的浸润和Foxp3、IL-17)及PD-1表达高于Hp阴性胃炎。2.Hp感染阳性胃炎胃黏膜中,萎缩性胃炎T淋巴细胞亚群及PD-1表达高于浅表性胃炎。
[Abstract]:Objective to detect the expression of T lymphocyte subsets and PD-1 in chronic gastritis (superficial gastritis, atrophic gastritis), and to explore the mechanism of gastric mucosal immune damage in chronic gastritis (gastritis, atrophic gastritis) infected with HP.To provide the basis for clinical application.Methods the patients signed informed consent after the examination and approval of the 97 th PLA Hospital Ethics Committee.Gastric mucosa specimens were collected from 40 cases of HP positive gastritis / HP positive superficial gastritis 20 cases of HP positive atrophic gastritis 20 cases of HP negative gastritis / HP negative superficial gastritis 20 cases of HP negative atrophic gastritis and 20 cases of HP negative atrophic gastritis under gastroscope.Immunohistochemical method was used to detect the expression of Foxp3mIL-17 and PD-1 on CD4 T cells. HP negative Hp-positive was determined by 14C breath test and HE staining. The positive group was positive in double positive group and negative group in double negative group.The cumulative optical density was measured by Image-pro plus image analysis system.Results the expression of CD4 T cell and CD8 T cell Foxp3 IL-17 and PD-1 in 1.Hp positive atrophic gastritis group was higher than that in HP negative atrophic gastritis group.There was a significant difference in the expression of Foxp3, IL-17 and PD-1 in CD4 T cells and CD8 T cells in patients with atrophic gastritis with HP positive, and the expression of Foxp3 IL-17 and PD-1 in HP positive atrophic gastritis group was higher than that in HP positive superficial gastritis group.There was a significant difference in the expression of CD4 T cell and CD8 T cell Foxp3IL-17 and PD-1 in gastric mucosa group with HP positive gastritis (superficial atrophy) than in HP negative gastritis (superficial atrophy) group, and the expression of Foxp3IL-17 and PD-1 in HP negative gastritis (superficial atrophy) group was higher than that in HP negative gastritis (superficial atrophy) group.The expression of IL-17 and PD-1 of CD4 T cells in the gastric mucosa of superficial gastritis with HP positive was higher than that in the gastric mucosa with HP negative superficial gastritis, and the expression of IL-17 and PD-1 in gastric mucosa of HP negative superficial gastritis was higher than that of HP negative superficial gastritis.The expression of CD8 T cells and Foxp3 in gastric mucosa of patients with HP positive superficial gastritis was significantly higher than that with HP negative superficial gastritis. There was no significant difference in the expression of CD8 T cells and Foxp3 in gastric mucosa of patients with HP negative superficial gastritis.Conclusion the infiltration of T lymphocyte subsets (CD 4 T cells) and the expression of Foxp3 (IL-17) and PD-1 in gastric mucosa of patients with 1.Hp positive gastritis are higher than those of HP negative gastritis. 2.The expression of T lymphocyte subsets and PD-1 in atrophic gastritis was higher than that in superficial gastritis.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R573.32

【参考文献】