大叶茜草素抑制大鼠CFSC-2G细胞活化和胶原合成

发布时间：2018-04-13 13:00

本文选题：大叶茜草素 + CFSC-G细胞　；参考：《中国病理生理杂志》2017年12期

【摘要】：目的:观察大叶茜草素(mollugin)对大鼠肝星状细胞系CFSC-2G活化和胶原合成的影响并探讨其分子机制。方法:小剂量(10μmol/L)过氧化氢(H_2O_2)诱导CFSC-2G细胞30 min后,再加入不同浓度(0、20、40、60和120μmol/L)的mollugin处理。MTT法检测细胞活力,real-time PCR和Western blot法分别检测核因子E2相关因子2(Nrf2)、血红素加氧酶-1(HO-1)、核因子κB(NF-κB)p65、Bcl-2、Bcl-x L、Bax以及肝星状细胞活化标志物α-平滑肌肌动蛋白和Ⅰ型胶原蛋白的mRNA和蛋白表达,并用Western blot法检测p38丝裂原活化蛋白激酶(p38MAPK)的磷酸化水平。结果:低剂量H_2O_2可以诱导CFSC-2G细胞活化,mollugin明显促进p38 MAPK磷酸化,上调Nrf2和HO-1的mRNA和蛋白表达,下调NF-κB p65、Bcl-2和Bcl-x L的mRNA和蛋白表达,抑制H_2O_2诱导活化的CFSC-2G细胞活力和胶原合成(P0.05)。结论:Mollugin可能通过上调Nrf2和HO-1并下调NF-κB p65和Bcl-2表达,抑制CFSC-2G细胞活化和胶原合成。
[Abstract]:Aim: to observe the effects of Rubidium grandiflorum on the activation of CFSC-2G and collagen synthesis in rat hepatic stellate cell line and to explore its molecular mechanism.Methods: low dose of 10 渭 mol / L H _ 2O _ 2 (H _ 2O _ 2) was used to induce CFSC-2G cells for 30 min.MRNA and protein expression of actin and type I collagen,The phosphorylation level of p38 mitogen activated protein kinase (p38 MAPK) was detected by Western blot method.Results: low dose H_2O_2 could induce the activation of CFSC-2G cells to promote the phosphorylation of p38 MAPK, up-regulate the expression of mRNA and protein of Nrf2 and HO-1, down-regulate the expression of mRNA and protein of NF- 魏 B p65- Bcl 2 and Bcl-x L, and inhibit the activation of CFSC-2G cells induced by H_2O_2 and the synthesis of collagen.Conclusion: by up-regulating the expression of Nrf2 and HO-1 and down-regulating the expression of NF- 魏 B p65 and Bcl-2, the activation and collagen synthesis of CFSC-2G cells may be inhibited by w: Mollugin.
【作者单位】：暨南大学附属第一医院消化科;暨南大学附属第四医院消化科;暨南大学中医学院;
【基金】：广东省中医药局资助项目(No.20141069)
【分类号】：R575.2

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