HLA-DP、DQ基因多态性与福建汉族人群乙型肝炎病毒感染及其不同结局的关联

发布时间：2018-04-14 18:36

本文选题：乙型肝炎病毒 + 单核苷酸多态性　；参考：《福建医科大学》2014年硕士论文

【摘要】：【目的】探讨HLA-DP、DQ基因单核苷酸多态性与福建汉族人群HBV感染及其不同结局的关系。【方法】采用PCR-SSP基因分型的方法对健康人群（n=204）、HBV自限性感染人群（n=255）、慢性HBV感染人群（无症状携带者204例、慢性乙型肝炎136例、肝硬化及肝细胞癌各68例）进行rs3077、rs9277535、rs2647050位点的多态性检测。【结果】健康对照组、自限性感染组和无症状HBV携带者组3个SNP位点基因型均符合Hardy-Weinberg遗传平衡。健康对照组、自限性感染组与慢性感染组相比，二元Logistic回归分析校正性别与年龄影响因素后，，rs3077、rs9277535位点A等位基因在显性和加性模型下均有统计学意义（P0.05），而rs2647050位点A等位基因在显性和加性模型无统计学意义（P0.05），单倍型GGA、AGA、AAA与HBV感染存在相关性（P0.05）。以无症状携带者为对照组，分别与慢性乙型肝炎组、肝硬化组及肝细胞癌组相比，以上3个SNP与HBV感染结局均无统计关联（P0.05），也无单倍型与HBV感染结局存在关联（P0.05）。【结论】HLA-DP基因多态性与慢性HBV感染存在相关性，rs3077A、rs9277535A是慢性HBV感染的保护因素。HLA-DP、DQ基因单核苷酸多态性与HBV感染不同结局之间无相关性。【目的】综合评价HLA-DP基因多态性与中国人群患肝癌风险的相关性。【方法】系统检索PubMed、FMJS、CNKI及万方数据库有关HLA-DP基因多态性与肝癌相关性的病例对照研究，按纳入标准选择文献并提取相关信息，应用Stata12.0软件进行Meta分析。【结果】本研究共纳入HLA-DP基因多态性与HBV相关肝癌相关文献3篇。Meta分析结果显示，rs3077A在显性模型下可降低患肝癌的风险（AA+AG vs GG：OR=0.83，95%CI：0.74～0.93），而rs9277535与患肝癌风险无相关性（AA+AGvs GG：OR=0.95，95%CI：0.85～1.06）。【结论】通过Meta分析，本研究发现HLA-DPA1基因rs3077A在显性模型下可降低患肝癌的风险。
[Abstract]:[objective] to investigate the relationship between HLA-DPnDQ gene single nucleotide polymorphism (SNP) and HBV infection and its different outcomes in Fujian Han population.[methods] PCR-SSP genotyping was used to study the prevalence of PCR-SSP infection in healthy persons (204 asymptomatic carriers, 136 chronic hepatitis B), and in patients with chronic HBV infection (204 asymptomatic carriers and 136 chronic hepatitis B).The polymorphism of rs3077 rs9277535rs2647050 was detected in 68 cases of liver cirrhosis and 68 cases of hepatocellular carcinoma respectively.[results] three SNP locus genotypes in healthy control group, self limited infection group and asymptomatic HBV carrier group all accord with Hardy-Weinberg genetic balance.In the healthy control group, the self-limited infection group was compared with the chronic infection group,A allele at rs3077 rs9277535 was statistically significant in both dominant and additive models by binary Logistic regression analysis, while the rs2647050 locus A allele had no statistical significance in dominant and additive models.There was a correlation between AGAA A and HBV infection (P0.05A).Compared with chronic hepatitis B group, liver cirrhosis group and hepatocellular carcinoma group, there was no statistical correlation between SNP and HBV infection outcome, and no haplotype was associated with HBV infection outcome.[conclusion] there is a correlation between the polymorphism of HLA-DP gene and chronic HBV infection. There is no correlation between the single nucleotide polymorphisms of HLA-DPnDQ gene and the different outcomes of HBV infection, rs3077A rs9277535A is the protective factor of chronic HBV infection.[objective] to evaluate the association of HLA-DP gene polymorphism with the risk of liver cancer in Chinese population.[methods] A case-control study on the association between HLA-DP gene polymorphism and hepatocellular carcinoma was carried out in PubMedus FMJSN CNKI and Wanfang database. According to the inclusion criteria, literature was selected and relevant information was extracted. Meta analysis was carried out with Stata12.0 software.[results] A total of 3 references related to HLA-DP gene polymorphisms and HBV related hepatocellular carcinoma were included in this study. The results of meta-analysis showed that rs3077A could reduce the risk of liver cancer under dominant model and reduce the risk of HCC by AA AG vs GG: OR0.8395CIW 0.7495 CIW 0.740.93, but rs9277535 had no correlation with the risk of HCC.[conclusion] by Meta analysis, we found that HLA-DPA1 gene rs3077A can reduce the risk of liver cancer in dominant model.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.62

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