重组幽门螺杆菌菌毛蛋白FLaA IgY乙基纤维素微囊对胃炎小鼠的治疗作用

发布时间：2018-04-17 08:35

本文选题：幽门螺杆菌 + 重组菌毛蛋白Fla　；参考：《中国药学杂志》2015年01期

【摘要】：目的研究重组幽门螺杆菌菌毛蛋白Fla A Ig Y-硫糖铝乙基纤维素微囊对幽门螺杆旋菌(Helicobacter pylori)感染小鼠的治疗和清除效果。方法采用有机溶剂蒸发技术制备经硫糖铝处理后的Ig Y乙基纤维素微囊。模拟胃部环境,检测在不同p H的正常胃蛋白酶浓度(0.02 mg·m L-1)和异常胃蛋白酶浓度(0.04 mg·m L-1)的条件下不同浓度硫糖铝对r Fla A Ig Y的保护效果。将r Fla A Ig Y-硫糖铝微囊按0.8、1.6、3.2及6.4 mg·g-1·d-14种不同剂量灌服实验组小鼠,通过检测治疗前后血清中抗幽门螺杆旋菌抗体水平以及胃黏膜的尿素酶水平,评价不同浓度r Fla A Ig Y对小鼠胃部幽门螺杆旋菌的治疗和清除效果。结果结果表明,40%的硫糖铝处理过的Ig Y在p H 3.0条件下的模拟胃液中能维持77.87%~82.14%的活性达8 h,而在p H 2.0的模拟胃液条件中处理8 h能保持57.76%~63.28%,在p H 1.0模拟胃液中处理8 h能保持21.52%~22.79%。制备的r Fla A Ig Y-硫糖铝乙基纤维素微囊的包封率为83.79%,5 h体外释放率为95.92%。各组小鼠经r Fla A Ig Y微囊治疗后血清中幽门螺杆旋菌抗体水平与阳性对照组相比均显著下降(P0.001)。当使用剂量为0.8 mg·g-1·d-1时,对幽门螺杆旋菌的清除率为50%,当剂量为1.6 mg·g-1·d-1时,幽门螺杆旋菌清除率为70%,当剂量为3.2 mg·g-1·d-1时,H.pylori清除率为80%,当剂量达到6.4 mg·g-1·d-1时,H.pylori的清除率可达100%。结论 40%的硫糖铝对r Fla A Ig Y具有很好的保护作用,可以作为r Fla A Ig Y的保护剂。r Fla A Ig Y-硫糖铝乙基纤维素微囊具有良好的缓释效果,经口服对小鼠胃部幽门螺杆旋菌感染具有良好的治疗和清除效果。
[Abstract]:Objective to study the efficacy of recombinant Helicobacter pylori pili protein Fla A Ig Y- thioaluminum ethyl cellulose microcapsules in the treatment and clearance of Helicobacter pylori infected mice.Methods Ig Y ethyl cellulose microcapsules treated with sucralfate were prepared by organic solvent evaporation.The protective effects of sucralfate at different concentrations of sucralfate on r Fla A Ig Y were measured under the conditions of normal pepsin concentration (0.02 mg ml -1) and abnormal pepsin concentration (0.04 mg ml -1).R Fla A Ig Y- sucralfate microcapsules were administered with different doses of 0.8mg / g ~ (-1) and 6.4 mg / g ~ (-1) 路d ~ (- 14) respectively. The levels of anti-helicobacter pylori antibodies in serum and urease levels in gastric mucosa were measured before and after treatment.To evaluate the efficacy of different concentrations of r Fla A Ig Y in the treatment and clearance of Helicobacter pylori in the stomach of mice.The results showed that 40% sucralfate treated IGY could maintain 77.87% activity of 82.14% in simulated gastric juice under pH 3.0 and 57.7663.28% in pH 2.0 simulated gastric juice for 8 h.After 8 hours treatment, 21.52% and 22.79% can be maintained.The encapsulation efficiency of r Fla A Ig Y- thioaluminium-ethyl cellulose microcapsules was 83.79 and the release rate in vitro was 95.92 for 5 h.After treatment with r Fla A Ig Y microcapsule, the antibody level of Helicobacter pylori in the serum of each group was significantly lower than that of the positive control group (P 0.001).The clearance rate of Helicobacter pylori was 50 when the dosage was 0.8 mg g -1 d-1, 70% when the dose was 1.6 mg g -1 d-1, 80% when the dose was 3.2 mg g -1 d-1, and when the dose reached 6.4 mg g -1 d-1, the clearance rate of H. pylori reached 100%.Conclusion 40% sucralfate has a good protective effect on r Fla A Ig Y, and can be used as a protective agent for r Fla A mg Y. R Fla A Ig Y ethyl cellulose microcapsule has a good sustained release effect.Oral administration of Helicobacter pylori infection in the stomach of mice has a good treatment and clearance effect.
【作者单位】：广西师范大学药用资源化学与药物分子工程国家重点实验室培育基地;深圳宝舜泰生物医药股份有限公司;
【基金】：国家自然科学基金资助项目(81401912) 广西自然科学基金资助项目(2013GXNSFBA019159) 广西医药产业人才小高地资助项目(1312) 广西科学研究与技术开发资助项目(13349002,13278004) 深圳市科创委深圳市战略性新兴产业发展专项基金资助项目(CXZZ20120823103224373)
【分类号】：R573.3

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