不同剂量桃仁提取物对急性胰腺炎大鼠肠道黏膜屏障功能及免疫功能的作用

发布时间：2018-04-19 00:31

本文选题：桃仁提取物 + 急性胰腺炎　；参考：《中国免疫学杂志》2015年03期

【摘要】：目的:研究不同剂量桃仁提取物对急性胰腺炎大鼠肠道黏膜屏障功能及免疫功能的作用。方法:48只大鼠制备SAP模型后随机分为模型对照组、桃仁提取物低剂量、中剂量和高剂量组,每组12只。另取12只大鼠作为假手术组,造模麻醉苏醒即开始干预,桃仁提取物低剂量组、中剂量组和高剂量组分别灌胃给予桃仁提取物0.12、0.248和0.36 g/kg,假手术组及模型组给予等体积蒸馏水灌胃,各组灌胃均1次/6 h,连续4次。给药后24 h应用10%水合氯醛麻醉各组大鼠,打开胸腔和腹腔,腹主动脉分别抽取5 ml血样于EDTA抗凝管和非抗凝管内,分别应用荧光直接标记法和流式细胞仪进行CD4+、CD8+和Treg细胞测定,采用免疫比浊法测定Ig A、Ig G和Ig M,采用EPS-G7底物法测定血清淀粉酶水平,采用酶学分光光度法检测血清D-乳酸水平,采用活性比色法测定血清二胺氧化酶;取小肠组织HE染色后采用光学显微镜进行病理学检查;取小肠组织采用放射免疫法进行s Ig A测定以及采用RT-PCR法进行TLR4和NF-κBp65 mRNA测定。结果:(1)中剂量和高剂量组大鼠血清淀粉酶、D-乳酸和二胺氧化酶水平均较低剂量组大鼠显著降低(P0.01),小肠黏膜s Ig A较低剂量组显著升高(P0.01),并且高剂量组和中剂量组差异具有统计学意义(P0.01);(2)桃仁提取物中剂量和高剂量组血液CD4+、CD4+/CD8+较低剂量组大鼠显著升高(P0.01),CD8+、Treg细胞较低剂量组大鼠显著降低(P0.01),并且高剂量组和中剂量组差异均具有统计学意义(P0.01);(3)桃仁提取物中剂量和高剂量组血清Ig A、Ig G、Ig M较低剂量组大鼠显著升高(P0.01),并且高剂量组和中剂量组差异均具有统计学意义(P0.01);(4)假手术组大鼠小肠黏膜无显著损伤,模型对照组大鼠小肠黏膜显著损伤,低剂量组大鼠小肠黏膜病理情况与模型组基本相似,中剂量和大剂量组大鼠小肠黏膜损伤显著降低;(5)桃仁提取物中剂量和高剂量组小肠组织TLR4和NF-κBp65 mRNA较低剂量组大鼠显著降低(P0.01),并且高剂量组和中剂量组差异均具有统计学意义(P0.01)。结论:桃仁提取物对急性胰腺炎大鼠肠道屏障功能具有保护作用,并且显著改善急性胰腺炎大鼠的免疫功能。
[Abstract]:Aim: to study the effects of different doses of peach kernel extract on intestinal mucosal barrier function and immune function in rats with acute pancreatitis.Methods SAP model was established in 48 rats. The rats were randomly divided into control group, low dose group, middle dose group and high dose group with 12 rats in each group.Another 12 rats were taken as sham-operation group, the anaesthesia recovery of model group began to intervene, the low dose group of peach kernel extract,The middle dose group and the high dose group were given 0.12g / kg and 0.36 g / kg of peach kernel extract respectively. The sham operation group and the model group were given the same volume distilled water. The rats in each group were given intragastric perfusion for 1 / 6 h for 4 consecutive times.24 hours after administration, 10% chloral hydrate was used to anesthetize the rats in each group. The thoracic cavity and abdominal cavity were opened, and 5 ml blood samples were taken from abdominal aorta in EDTA anticoagulant tube and non-anticoagulant tube respectively.CD4 CD8 and Treg cells were detected by fluorescence direct labeling method and flow cytometry respectively. The levels of serum amylase were measured by immunoturbidimetric assay (Ig G) and IgM (IgM). The serum amylase levels were measured by EPS-G7 substrate method.The level of serum D-lactic acid was detected by enzymatic spectrophotometry, the serum diamine oxidase was determined by active colorimetry, the tissues of small intestine were stained by HE and the pathology was examined by optical microscope.Small intestinal tissues were detected by radioimmunoassay (RIA) and TLR4 and NF- 魏 Bp65 mRNA (NF- 魏 Bp65 mRNA) by RT-PCR method.Results the levels of serum amylase D-lactic acid and diamine oxidase in the middle and high dose groups were significantly lower than those in the low dose group, and the levels of SIG A in small intestine mucosa were significantly higher than those in the low dose group, and the levels of high and medium dose groups were significantly higher than those in the low dose group.The difference was statistically significant (P 0.01)) the blood CD4 CD 4 / CD 8 in the middle dose group and the high dose group were significantly higher than those in the low dose group. The number of P0.01T 8 / CD 8 Treg cells in the low dose group was significantly lower than that in the low dose group, and there was a significant difference between the high dose group and the middle dose group, and the difference between the high dose group and the middle dose group was also found in the difference between the high dose group and the middle dose group.Compared with the low dose group, the serum Ig G G G M increased significantly, and the difference between the high dose group and the middle dose group was statistically significant (P 0.01). There was no significant damage to the intestinal mucosa in the sham operation group, and the difference between the high dose group and the middle dose group was statistically significant (P 0.01), and the difference between the high dose group and the middle dose group was statistically significant (P 0.01), and there was no significant damage to the intestinal mucosa in the sham operation group.The pathological changes of intestinal mucosa in the low dose group were similar to those in the model group.The TLR4 and NF- 魏 Bp65 mRNA of small intestine tissue in the middle and high dose groups were significantly lower than those in the low dose group, and the difference between the high dose group and the middle dose group was statistically significant (P 0.01).Conclusion: Peach kernel extract has protective effect on intestinal barrier function in rats with acute pancreatitis and improves the immune function of acute pancreatitis rats.
【作者单位】：河北省沧州市人民医院普外二科;
【基金】：河北省中医药管理局科研计划资助项目《桃仁提取物在急性胰腺炎致肠道微循环障碍中的作用及机制研究》(No.2013141)
【分类号】：R576

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