SIRT1和APOC3基因单核苷酸多态性与非酒精性脂肪性肝病的相关性

发布时间：2018-04-19 07:22

本文选题：非酒精性脂肪性肝病 + 沉默信息调节因子　；参考：《卫生研究》2017年04期

【摘要】：目的探讨沉默信息调节因子1(SIRT1)基因rs12778366位点和载脂蛋白C3(APOC3)基因rs2854116位点单核苷酸多态性(SNP)与非酒精性脂肪性肝病(NAFLD)发病易感性的关系。方法数据源于2015年苏州市相城区医院体检人群,共收集病例560人,根据B超结果,筛选纳入NAFLD病例132例,随机选取正常对照人群252例,采集血液样本,分析总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶和血尿酸的血生化指标,及身高、体重、腰围、臀围、血压一般指标,全血中提取DNA,采用聚合酶链反应(PCR)、Mass ARRAY时间飞行质谱技术分析SIRT1 rs12778366和APOC3rs2854116位点基因型。结果 SIRT1 rs12778366位点TC+CC基因型携带者相对于TT基因型携带者,发生NAFLD的OR=1.126(95%CI 0.673~1.886),P0.05。与APOC3 rs2854116位点CC基因型携带者相比较,TC+TT基因型携带者发生NAFLD的OR=1.044(95%CI 0.601~1.814),P0.05。校正性别、年龄、体质指数的混杂因素后OR均无明显改变(P0.05)。多因素Logistic回归分析显示:甘油三酯、体质指数、空腹血糖、腰围和尿酸是NAFLD发病可能的独立危险因素(P0.05),而SIRT1 rs12778366和APOC3 rs2854116位点基因多态性与NAFLD的发病风险无明显关联。结论 SIRT1 rs12778366和APOC3 rs2854116位点基因多态性与NAFLD的发生无明显相关性,均不会影响NAFLD的发病风险。
[Abstract]:Objective to investigate the relationship between the single nucleotide polymorphisms (SNPs) of rs12778366 locus and apolipoprotein C3 (APOC3) gene rs2854116 locus of silencing signaling factor 1 sirt1) gene and the susceptibility to NAF LDD of non-alcoholic fatty liver disease.Methods data were collected from a total of 560cases collected from the physical examination population of Xiangcheng District Hospital of Suzhou City in 2015. According to the results of B-mode ultrasound, 132 cases of NAFLD were selected and 252 cases of normal controls were randomly selected to collect blood samples and analyze total cholesterol (TC).Serum biochemical indexes of triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, fasting blood glucose, alanine aminotransferase, aspartate aminotransferase and serum uric acid, and height, weight, waist circumference, hip circumference,The genotypes of SIRT1 rs12778366 and APOC3rs2854116 loci were analyzed by polymerase chain reaction (PCR) mass ARRAY time-flight mass spectrometry (TF-MS).Results TCCC genotype carriers at SIRT1 rs12778366 locus were significantly higher than TT genotype carriers (P 0.05).Compared with CC genotype carriers of APOC3 rs2854116 locus, the OR=1.044(95%CI 0.601 and 1.814P0.05 of TCTT genotype carriers developed NAFLD.After adjusting for the mixed factors of sex, age and BMI, the OR had no significant change (P 0.05).Multivariate Logistic regression analysis showed that triglyceride, body mass index, fasting blood glucose, waist circumference and uric acid were independent risk factors for NAFLD, while SIRT1 rs12778366 and APOC3 rs2854116 locus polymorphism were not significantly associated with the risk of NAFLD.Conclusion there is no significant correlation between the polymorphism of SIRT1 rs12778366 and APOC3 rs2854116 loci and the occurrence of NAFLD, and neither of them will affect the risk of NAFLD.
【作者单位】：苏州大学公共卫生学院营养与食品卫生教研室;苏州市相城区第三人民医院;
【基金】：国家自然科学基金面上项目(No.81372980,81673150)
【分类号】：R575.5

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